首页|Highly exposed Cu active sites as efficient peroxidase mimics for colorimetric analysis

Highly exposed Cu active sites as efficient peroxidase mimics for colorimetric analysis

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Nanozymes are nanomaterials with intrinsic enzyme-mimic activity,but their large-scale application is generally limited by their low catalytic activity.Herein,we demonstrated that highly exposed Cu active sites on two-dimensional(2D)nitrogen-doped carbon(Cux/NC)can serve as efficient peroxidase-like(POD)catalysts with high atomic utilization.Specially,the uniformly distributed Cu active sites could react with H2O2 to produce singlet oxygen(1O2)under acidic conditions,which can efficiently oxidizes colorless 3,3',5,5'-tetramethylbenzidine(TMB)to blue oxidized TMB(oxTMB).Among various Cux/NC nanozymes studied,the Cu0.14/NC exhibited smaller maximum catalytic velocities(Vmax)and Menten constant(Km)for TMB and H2O2.Benefiting from the highly active peroxidase-like activity,the Cu0.14/NC nanozyme could be successfully applied for the hydroquinone(HQ)and ascorbic acid(AA)detection applications through the inhibitory effect of HQ and AA.More interestingly,α-glucosidase(α-Glu)detection sensing platform could be constructed based on HQ as a signal transmitter,with the detection range ranging from 0 to 12 U/L and the minimum detection limit being 0.68 U/L.This work provides not only an idea for the rational design of highly exposed Cu active sites but also fabricate an effective detection sensing platform for HQ,AA,and α-Glu detection.

NanozymeHighly exposed active sitesPeroxidase-likeα-glucosidaseSensing

Hengya Wei、Shushu Chu、Fangning Liu、Shengzhen Li、Yizhong Lu

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School of Materials Science and Engineering University of Jinan,Jinan 250022,China

National Natural Science Foundation of ChinaYoung Taishan Scholar ProgramIndependent Cultivation Program of Innovation Team of Jinan City

22172063tsqn2018120802021GXRC052

2024

10.1016/j.chphma.2023.12.001

化学物理材料(英文)

化学物理材料(英文)

ISSN：
年,卷(期)：2024.3(2)
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