RNA-Binding Proteome Analysis and Functional Explorations
Post-transcriptional regulations play a critical role in diverse biological processes,and a series of RNA-protein interactions are involved in their underlying mechanism.RNA-binding proteins(RBPs)are key participants and executors in entire life cycle of RNA,affecting the operation of biological processes and the maintenance of cellular homeostasis.The RNA-binding proteome profiling therefore provides crucial information for discovering biological markers and targets of diseases.An array of RBPs capturing methods have been developed in recent years,ranging from profiling binding proteins of specific RNA to unbiased global RBPs identification,and even to exploring the functional aspects of RBPs such as spatial distribution and/or post-translational modifications.This review will focus on tool development for RBP proteomics and functional proteome characterization,and finally discusses bottlenecks and future prospects of this exciting direction of RBP proteomics.
万方数据
维普
