Effects of Water-Accommodated Fraction in Crude Oil on Seed Germination and Physiological Indicators of Black Tartary Buckwheat
Water is an essential factor for plant growth,and plant growth is threatened by water pollution in oil-pro-ducing areas.In the present study,it was found that the effects on the seed germination and seedling growth of black tar-tary buckwheat varied significantly with different concentrations of water-accommodated fraction(WAF)in crude oil.When the concentration of WAF in crude oil increased from 0%to 6%,the hypocotyl length,root length,and germina-tion rate increased gradually and then showed a downward trend at the concentration of 6%to 20%.The changes in total protein content,malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,and proline(Pro)content in black tartary buckwheat were also significantly different under the stress of WAF in crude oil.The protein content de-creased,while the MDA content,SOD activity,and Pro content increased with the increase in WAF of crude oil,al-though the increase in SOD activity and Pro content was gradual.This study preliminarily discusses the effect of WAF in crude oil on the seed germination and seedling growth of black tartary buckwheat,which provides a foundation for under-standing the impact of crude oil-contaminated water on the yield and quality of buckwheat in the Longdong area.
Water-accommodated fraction in crude oilBlack tartary buckwheatSeed germinationSeedling growth
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