Investigation of Active Components for Pharyngitis Treatment Using Network Pharmacology and Molecular Docking Technology
Objective:This study aimed to investigate the mechanisms of action in the treatment of acute and chronic phar-yngitis using network pharmacology and molecular docking techniques,identifying the corresponding chemical components,tar-get proteins,and action pathways.Methods:We collected and screened data from the UniProt database,using"laryngitis"as a keyword to retrieve pharyngitis-related targets.A network diagram of"active ingredient-target"was constructed.Through protein interaction analysis,we established a protein interaction network and conducted pathway enrichment analysis to explore the mechanisms underlying pharyngitis.Results:After screening,we identified five key active components and predicted 20 poten-tial targets for treating acute and chronic pharyngitis.The main targets for the treatment of pharyngitis included glutathione S-transferase M1,cytochrome P450 3A4,tumor necrosis factor,cytochrome P450 family 2 subfamily A6,cold-sensing protein,glucuronosyltransferase 1,alcohol dehydrogenase 6(V),arachidonate 5-lipoxygenase,and N-acetyltransferase,among others.GO analysis and KEGG pathway analysis indicated that the signaling pathways associated with acute and chronic pharyngitis pri-marily involved lipid and atherosclerosis pathways,human cytomegalovirus infection pathways,hepatitis B pathways,and AGE-RAGE signaling pathways.Cconslusion:The potential mechanisms of treatment for acute and chronic pharyngitis may involve the regulation of multiple signaling pathways,such as CASP3,JUN,GSTM1,CYP3A4,and TNF,through the action of β-sitoster-ol and its components.
network pharmacologyraditrunkpharyngitismechanism of action
NETL
NSTL
万方数据
