Infection status and genomic characterization of hepatitis E virus and Wenzhou virus in rodents in Longyan City,Fujian Province
Objective To investigate infection status and genomic characteristics of Hepatitis E virus(HEV)and Wenzhou virus(WENV)in rodents in Longyan City of Fujian Province,in order to provide reference for the prevention and control of HEV and WENV.Methods Rodent intestinal tissue samples were collected in Zhangping City and Wuping County.The RNA-dependent RNA polymerase(RdRp)gene of HEV and Small segment(S)gene of WENV were ampli-fied by nested PCR.Positive PCR products were sent for sequencing.Genomic characterization was performed using bioinformatics software.Results A total of 141 rodents were captured in the current study.The positive rate of HEV was 7.8％.The infected animals were R.losea,R.tanezumi,and N.fulvescens,with infection rates of 22.2％(2/9),10.0％(8/80)and 2.9％(1/35),respectively.Sequence analysis showed that the HEV found in rodents in Longyan City was closely related to HEV found in rodent and shrew from Shenzhen City,whereas there was about 14％difference com-pared to HEV isolates from Hong Kong patients.WENV was not detected in any of the rodents.Conclusion This is the first study that detected HEV infection in rodents in Fujian Province,which has important implications for prevention and control.Although WENV infection was not detected in rodents in the current study,enhanced surveillance is still necessary to prevent potential WENV outbreaks.
Hepatitis E VrusWenzhou VirusRodentsVirus SurveillanceGene Characteristics
NETL
NSTL
万方数据
