Study on saponins in different parts and fruit pedicel of notoginseng
The similarities and differences of saponins composition in different parts of Panax notoginseng(Burk.)F.H.Chen(including rhizome,fibrous root,main root,stem and leaf,fruit pedicel and flower)were analyzed in this paper.The contents of Rb3,Fp2,Fc and Rd in the fruit pedicel were determined.HPLC method was used to judge the saponin differences in different parts of P.notoginseng,and to determine the contents of Rb3,Fp2,Fc and Rd in fruit pedicel.Agilent HC-C18 column(4.6×250 mm,5 μ m)was used with acetonitrile-water as mobile phase,gradient elution.The flow rate was 1.0 mL/min and detection wavelength was set at 203 nm.There were more triol saponins in underground parts and more diol saponins in aboveground parts of notoginseng.Linear relationship of ginsenoside Rd,Rb3 and notoginsenoside Fc,Fp2 were good in the range of 0.2-2.0 mg/mL(R2>0.9993).The content of Rb3,Fp2,Fc and Rd were 1.4%,0.68%,0.71%and 0.31%in fruit pedicel,respectively.The chemical compositions of aboveground and underground parts of P.notoginseng were quite different.Saponin compositions of fruit pedicel,flower,stem and leaf were very similar,so it can be preliminarily considered that fruit pedicel can replace stem and leaf as saponin source.Contents of Rd,Rb3 and Fp2 in fruit pedicel were determined for the first time and the HPLC method established in this study provided a simple,rapid and sensitive mean for quality control of fruit pedicel of notoginseng.
notoginsengfruit pedicelSaponins contentcomparison of different partsHPLC
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