Hypochloric acid(HClO),as one of the important small molecule reactive oxygen species(ROS)in the body,is the second signal molecule in cells and between cells.It is an indispensable active molecule in the organism to regulate physiological and pathological processes.Monitoring its level in the organism is crucial to understand its biological function and pathological effects.We have developed a novel fluorescent probe for specific recognition of ClO-,which is connected to a dicyano isoflurone with a large Stokes shift using tetrahydro-2-naphthone as the backbone.The detection of ClO-is achieved through the oxidative action of ClO-on the C=C double bond to form a five membered thiophene ring.The probe exhibits high selectivity and rapid response(within 3 min.)to the fluorescence response of ClO-,with a detection limit(0.02 nmol/L).This study provides a new mechanism for sensing ClO-,cell and live animal imaging,further demonstrating the promising application prospects of these probes in biosensing and biological imaging.
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