Analysis and identification of differentially expressed genes related to spleen immunity induced by cadmium exposure in mice
Objective This study aims to explore the effects of cadmium(Cd)exposure on spleen tissue damage and gene expression,and explore the underlying molecular mechanisms of cadmium immunotoxicity.Methods A single subcutaneous injection of CdCl2(5 μmol/kg)was used to expose the mouse model.The Synergy H4 hybrid microplate reader was used to detect malondialdehyde(MDA)content in spleen tissue and Ca2+content in human B lymphocytoma cells,and the differentially expressed genes in the spleen were identified by DNA microarray analysis.GO enrichment,KEGG sinaling,and Reactome pathway database were used to analyze the immune related functions of Cd exposure interference.RT-qPCR,immunofluorescence and Western blot were used to differentially expressed genes.Results Cd exposure interferes with the autophagic function of cells,causing Ca2+homeostasis imbalance and enhanced oxidative stress in tissues.There were 934 differentially expressed immune-related genes,including 687 up-regulated genes and 247 down-regulated genes.Cd-induced four key immune genes(Ppp3ca,Plcg2,Vav1,Cd247)regulated the innate and adaptive immune systems.Conclusion The expression changes of key immune regulatory genes(Ppp3ca,Plcg2,Vav1,and Cd247)induced by cadmium exposure may be the molecular mechanism of their immunotoxicity to mouse spleen.
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