Hydrogen sulfide antagonizes uranium-induced rats nephrotoxicity by alleviating carbonyl stress
Objective To investigate whether hydrogen sulfide(H2 S)can alleviate uranium-induced rats nephrotoxicity by antagonizing carbonyl stress.Methods SD male rats were divided into four groups:normal control group,GYY4137(H2 S donor)treatment group,uranium exposure group,and GYY4137 treatment group.The contents of creatinine and Blood urea nitrogen in urine were determined by microplate method and colorimetry.Kidney tissue damage was examined by HE staining.Kidney cell apoptosis was detected by Tunel method.Thiobarbituric Acid Reactive Substances(TBARS)and 4-Hydroxynonenal(4-HNE)contents were determined by enzyme-linked immunosorbent assay and MDA content was determined by thio Barbituric acid method.The contents of protein carbonylation(PCO)and 8-hydroxy Deoxyguanosine(8-OhdG)were measured by spectrophotometry.Western blots was used to detect the expression of aldehyde metabolic enzymes including alcohol dehydrogenase 1(ADH1),aldehyde dehydrogenase gene 2(ALDH2),aldo-keto-reductase-7A1(AKR7A1).Results Uranium exposure decreased the contents of creatinine and Blood urea nitrogen,led to structural damage of renal tissue,induced kidney cells apoptosis,increased the content of active aldehydes,PCO,8-OhdG,and reduced the expression of aldehyde metabolic enzymes.The difference is statistically significant(P<0.05).However,GYY4137 administration reversed the above experimental indicators of uranium effect(P<0.05).Conclusion H2 S can induce the expression of aldehyde metabolism enzymes,promote active aldehyde metabolism,and alleviate carbonyl stress,resulting in antagonize uranium-triggered rats nephrotoxicity.
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