Protective effect of leonurine on acute alcohol-induced liver injury
Objective To investigate the protective effects of leonurine against acute alcohol-induced liver injury.Methods The experimental design involved the random allocation of C57BL/6J mice into five groups:control group,model group,low-dose leonurine group,high-dose leonurine group,and Bifendate group,with 8 mice in each group.The leonurine and Bifendate groups were received their designatd doses through oral gavage once daily for a continuous period of 10 d.In contrast,the control and model groups were given an equivalent volume of saline solution during the same timeframe.12 h after the final dose,all groups except the control were given 56%alcohol content Beijing Erguotou by oral gavage for three consecutive days,with a 12-hour interval between each administration.After the final administration of Beijing Erguotou,the mice were fasted for 4 h before euthanasia,and their livers were weighed to calculate the liver coefficient.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),and malondialdehyde(MDA)were measured.Hepatic pathological changes were observed via hematoxylin-eosin staining,and relevant proteins were detected by Western blot.Results In the model group,levels of ALT,AST,TG,MDA,and liver indices were significantly higher compared to the control group,with a statistically significant difference(P<0.05).The levels of ALT,AST,TG,MDA in both low-dose and high-dose leonurine groups were lower than those in the model group(P<0.05).Hepatic pathological examination and Oil Red O staining indicated that leonurine reduced ethanol-induced liver tissue damage in mice(P<0.05).Conclusion leonurine demonstrates a protective effect against acute alcohol-induced liver injury.
NETL
NSTL
万方数据
