Huoxin pill(concentrated pill)alleviates atherosclerosis in ApoE-/-mice by down-regulating TLR4/TNF-α expression
Objective To investigate the effect and mechanism of Huoxin pill(concentrated pill)on atherosclerosis(As)in apolipoprotein E knockout(ApoE-/-)mice.Methods ApoE-/-mice were fed with high-fat diet to replicate As model.The mice were treated with different concentrations of Huoxin pills and Toll-like receptor 4(TLR4)inhibitor.The mice were randomly divided into control group,Huoxin pill treatment group,TLR4 inhibitor treatment group,and Huoxin pill and TLR4 inhibitor co-treatment group.Hematoxylin-eosin(HE)staining and Masson staining were used to observe the pathological changes of aortic sinus of As model mice.Oil red O(ORO)staining was used to observe the lipid accumulation in aortic sinus and aorta of As model mice.Immunohistochemistry was used to detect the expression of TLR4 and tumor necrosis factor-α(TNF-α)in aortic sinus of As model mice.Results Compared with the control group,Huoxin pills significantly reduced the aortic sinus lesions,reduced the lipid accumulation in the aortic lesions and the degree of aortic sinus stenosis of As model mice,with a statistically significant difference(P<0.05).In addition,Huoxin pills also down-regulated the expression of TLR4 and TNF-α in aortic sinus of As model mice(P<0.05),and the effect of TLR4 inhibitor and Huoxin pills had a synergistic effect.Conclusion Huoxin pills can alleviate As in ApoE-/-mice and its mechanism may be related to down-regulating the expression of TLR4 and TNF-α and regulating the inflammatory response.
Huoxin pillatherosclerosisToll like receptor 4tumor necrosis factor-α
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