TO901317经LXRα/NF-κB抑制MCF-7乳腺癌细胞迁移
TO901317 inhibits the migration of MCF-7 breast cancer cells through LXRα/NF-κB
涂剑 1王彦翔 1周志刚 2杨萍 3刘晓旺 4余平4
作者信息
- 1. 桂林医学院 药学院,桂林 541199
- 2. 桂林医学院 第二附属医院,桂林 541199
- 3. 桂林医学院 临床医学院,桂林 541199
- 4. 南华大学药学院,衡阳 421001
- 折叠
摘要
目的 探究肝X受体(LXR)激动剂TO901317 对MCF-7 人乳腺癌细胞迁移能力的作用及机制.方法 体外培养MCF-7 细胞.先运用不同浓度的TO901317 处理MCF-7 细胞 24 h,然后通过LXRα siRNA转染或核因子κB(NF-κB)抑制剂PDTC处理细胞,划痕愈合实验检测MCF-7 细胞迁移能力的改变,同时运用蛋白免疫印迹法检测 LXRα、NF-κB p65 与 IκBα 的表达.结果 随着TO901317 处理浓度的增加,MCF-7 细胞的迁移能力明显得到抑制,差异具有统计学意义(P<0.05);同时LXRα与IκBα的表达逐渐增强,而NF-κB p65 的表达则显著降低.LXRα siRNA可显著延缓TO901317 的上述作用,PDTC处理则进一步增强TO901317 对乳腺癌细胞迁移的抑制作用.结论 TO901317 可激活LXRα,下调NF-κB p65、上调IκBα的表达,抑制乳腺癌细胞迁移.
Abstract
Objective To explore the effect and mechanism of liver X receptor(LXR)agonist TO901317 on the migration ability of MCF-7 human breast cancer cells.Methods MCF-7 cells were cultured in vitro.At first,MCF-7 cells were treated with TO901317 with different concentrations for 24 h,and then the cells were transfected with LXRα siRNA or treated with PDTC,a nuclear factor κB(NF-κB)inhibitor.The changes of migration ability of MCF-7 cells were detected by scratch healing experiment,and the expressions of LXRα,NF-κB p65 and IκBα were detected by Western blot.Results With the increase of TO901317 concentration,the migration ability of MCF-7 cells was obviously inhibited,and the difference was statistically significant(P<0.05).At the same time,the expressions of LXRα and IκBα increased gradually,while the expression of NF-κB p65 decreased significantly.LXRα siRNA can significantly delay the above-mentioned effects of TO901317,and PDTC treatment can further enhance the inhibitory effect of TO901317 on breast cancer cell migration.Conclusion TO901317 can activate LXRα,down-regulate NF-κB p65,up-regulate the expression of IκBα,and inhibit the migration of breast cancer cells.
关键词
TO901317/肝X受体α/核因子κB/MCF-7乳腺癌细胞/细胞迁移Key words
TO901317/liver X receptor α/NF-κB/MCF-7 breast cancer cells/cell migration引用本文复制引用
基金项目
国家自然科学基金(82060662)
国家自然科学基金(81541163)
广西自然科学基金(2022JJA140776)
广西自然科学基金(2022JJA140639)
国家级大学生创新创业训练计划(202110601011)
出版年
2024
NETL
NSTL
万方数据