Protective effects of epsilonine on ox-LDL-induced pyroptosis of THP-1-derived macrophages
Objective To investigate the protective effects of leonurine(LEO)on pyroptosis induced by oxidized low-density lipoprotein(ox-LDL)in THP-1-derived macrophages.Methods MTT assay was used to detect the viability of THP-1 macrophages/THP-1-derived foam cells after treatment with different concentrations of LEO.Oil Red O staining was used to assess the intracellular lipid accumulation,meanwhile the contents of intracellular total cholesterol(TC),triglycerides(TG),interleukin-1β(IL-1β),interleukin-18(IL-18),and lactate dehydrogenase(LDH)in the cell supernatan were detected.Western blot was used to detect the expression level of proteins related to cellular pyrolysis.The level of reactive oxygen species(ROS)was determined by flow cytometry.Results As the concentration of LEO increased,there was a positive correlation between cell activity and LEO concentration.LEO intervention decreased the intracellular TC and TG levels,and reduced the intracellular lipid accumulation.LEO decreased the expression of cellular pyroptosis-related proteins,including NLRP3,N-GSDMD,Cleaved Caspase-1.At the same time,treatment with LEO reversed the ox-LDL-induced increase in ROS production.Conclusion LEO decreases lipid accumulation in THP-1 macrophages induced by ox-LDL and inhibits the pyroptosis induced by ox-LDL in THP-1-derired macrophage.
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