Quantitative proteomic study on anti-colorectal cancer effects of triterpenoid saponins of Cyclocarya paliurus
Objective To investigate the anti-human colorectal cancer cell activity of Cycas cycas triterpenoid saponins Cypaliuruside K in vitro,and to analyze the mechanism of anti-human colorectal cancer cell activity of Cypaliurusidek.Methods Cypaliuruside K,isolated from Cyclocarya paliurus,was used as an experimental drug to intervene SW480 tumor cells.The experiment was divided into control group and drug group,and the total protein in each group was extracted for tandem mass spectrometry(TMT)proteomic analysis.Results There were 402 differentially expressed proteins.Subcellular localization analysis showed that most of the differentially expressed proteins were located in mitochondria,cytoplasm and cell membrane.Gene ontology(GO)analysis showed that differential proteins were mainly involved in DNA replication,protein synthesis and glucose metabolism.The analysis results of Kyoto Encyclopedia of Genes and Genomes(KEEG)showed that differential proteins were mainly involved in cell metabolism,genetic information transmission,biological system pathways and other related signaling pathways.Conclusion Based on the 8 selected proteins closely related to anti-human colorectal cancer cells,quantitative proteomics preliminatively reveals that Cypaliuruside K may increase the expressions of MAPK8,SMAD2,PMAIP1,BBC3,JUN,MAPK1,NRAS and decrease the expressions of CYCS and achieves anti-SW480 tumor cell effect in vitro.
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