Effects and mechanism of Ardisia gigantisfolia extract on pancreatic cancer
Objective To observe the effect of Ardisia gigantisfolia extract on the proliferation,migration,invasion and apoptosis of pancreatic cancer cell line PANC-1 cells and explore its mechanism.Methods The MTT assay was used to test the proliferation of PANC-1 cells.The morphological change of PANC-1 cells was observed.Scratch and Transwell migration/invasion assays were performed to test the migration and invasion abilities of PANC-1 cells.Western blot analysis was conducted to examine the levels of protein expressions of p65,P-p65,Caspase-3,Caspase-9,Cleaved-Caspase-3,Cleaved-Caspase-9,Bcl-2,and Bax in PANC-1 cells.Results The proliferation,migration,and invasion of PANC-1 cells were inhibited by Ardisia gigantisfolia extract.The protein expression levels of P-p65,Caspase-3,Caspase-9 and Bcl-2 in PANC-1 cells were down-regulated by Ardisia gigantisfolia extract.The protein expressions of Cleaved-Caspase-3,Cleaved-Caspase-9 and Bax in PANC-1 cells were up-regulated by Ardisia gigantisfolia extract.Conclusion Ardisia gigantisfolia extract inhibits the proliferation,migration and invasion of PANC-1 cells,which the mechanism may be related to the upregulation of Bax,downregulation of Bcl-2,inhibition of p65 phosphorylation,promotion of Caspase-3 and Caspase-9 activation,and induction of apoptosis in PANC-1 cells induced by Ardisia gigantisfolia extract.
Ardisia gigantifolia extractpancreatic cancerproliferationmigration and invasionapoptosis
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