Lanatoside C inhibits the proliferation of HepG2 cells and down-regulates Akt expression
Objective To observe the effect of Lanatoside C on the proliferation in hepatocellular carcinoma cell HepG2 and to explore its potential molecular mechanism.Methods HepG2 cells were treated with various concentrations of Lanatoside C,and the effect of Lanatoside C on the proliferation of HepG2 cells was evaluated by cell proliferation and clonal formation experiments.Flow cytometry was used to analyze the cell cycle.Western blot assay was used to test the protein expression levels of cell cycle related proteins p21,p27,CDK4,p-Akt and Akt.Results The proliferation of HepG2 cells was significantly inhibited by Lanatoside C in a dose-dependent manner.Compared with the control group,the clonogenesis rate of HepG2 cells was significantly decreased by Lanatoside C.Flow cytometry results showed that Lanatoside C blocked HepG2 cells in G0/G1 phase.Western blot results showed that the protein expressions of p21 and p27 in HepG2 cells were significantly up-regulated by Lanatoside C,and the protein expressions of p-Akt and Akt were significantly down-regulated.Conclusion Lanatoside C inhibites the proliferation and induces the cell cycle arrest in HepG2 cells,which the mechanism may be related to the inhibition of Akt protein expression and phosphorylation induced by Lanatoside C,as well as the up-regulation of cell cycle regulatory proteins p21 and p27 induced by Lanatoside C.
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