Effects of epigallocatechin-3-gallate coupled with mechanical stimulation on the proliferation and differentiation of osteoblast
Objective To investigate the effect of epigallocatechin gallate(EGCG)coupled with mechanical stimulation(MS)on the proliferation and differentiation in osteoblasts.Methods The osteoblasts were divided into control group,EGCG group,MS group,and EGCG+MS group.The proliferation of osteoblasts was detected by MTT assay.The mineralisation of osteoblasts were detected by alizarin red staining method.The mechanical load was applied to the cells by a four-point bending mechanical loading device,and the Alkaline Phosphatase(ALP)Test Kit was used to detect the ALP activity.The protein expressions of runt-related transcription factor-2(Runx-2)and collagen type I(Col-I)in osteoblasts were detected by western blot.Results EGCG at a concentration of 30 μmol/L had the most pronounced proliferative effect on osteoblasts,with a statistically significant difference(P<0.05).The number of mineralised nodules in the EGCG group was greater than that in the control group.Compared with the single EGCG or MS groups,the ALP activity,in the EGCG+MS group was significantly increased and the protein expressions of Runx-2 and Col-I in the EGCG+MS group were significantly increased(P<0.05).Conclusion EGCG-coupled mechanical stimulation enhances the ALP activity,up-regulates the expressions of Runx-2 and Col-I proteins in osteoblasts,and promotes osteoblasts'proliferation and differentiation.
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