Exploring the influencing mechanisms of hirudin on HK-2 cells based on transcriptomics
Objective To explore the effects and mechanisms of hirudin on human kidney-2 cell(HK-2 cells)using transcriptomics techniques.Methods The CCK-8 method was used to detect the viability of HK-2 cells,and transcriptome sequencing was performed in the solvent control group and hirudin group.The gene expression levels in different samples were calculated using the RSEM method,and differential gene screening was performed using the DEseq2 method with a screening criterion of|log2FC|≥1 and P<0.05.Subsequently,enrichment analysis was performed based on differentially expressed genes.Results The hirudin(5 mg/mL)had the best effect on enhancing the viability of HK-2 cells.Transcriptomic studies found that compared with normal HK-2 cells,there were 1723 genes were differentially expressed in HK-2 cells treated with hirudin.GO and KEGG analysis results showed that the regulation of inflammatory pathways was the main mechanism by which hirudin increased HK-2 cels viability.Conclusion Hirudin increases the viability of HK-2 cells,and its mechanism may be involved the regulation of inflammatory pathways.
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