PBK/TOPK在类风湿关节炎患者单核细胞中的表达及临床意义
Expression and clinical significance of PBK/TOPK in monocytes of patients with rheumatoid arthritis
王琪 1覃泱 2臧念可 3刘瑜 4辛林伟 5魏兵3
作者信息
- 1. 桂林医学院公共卫生学院,桂林 541199;广西肿瘤免疫与微环境调控重点实验室,桂林 541199
- 2. 桂林医学院附属医院,桂林 541001
- 3. 广西肿瘤免疫与微环境调控重点实验室,桂林 541199;桂林医学院附属医院,桂林 541001
- 4. 广西肿瘤免疫与微环境调控重点实验室,桂林 541199
- 5. 桂林医学院第二附属医院,桂林 541199
- 折叠
摘要
目的 研究PDZ结合激酶/T-淋巴因子激活的杀伤细胞来源的蛋白激酶(PBK/TOPK)在类风湿关节炎(RA)患者单核细胞的表达及临床意义.方法 以47 例RA患者、20 例健康体检者为研究对象,采用流式细胞术检测外周血单个核细胞(PBMC)中PBK/TOPK的表达.结果 PBK/TOPK在RA患者CD14+单核细胞表达水平高于健康体检者,差异具有统计学意义(P<0.05).RA患者CD14+单核细胞PBK/TOPK低表达组CD14+单核细胞在 PBMC 中所占比例、白细胞(WBC)计数、类风湿因子(RF)、免疫球蛋白A(IgA)、免疫球蛋白G(IgG)的水平均高于PBK/TOPK高表达组,差异具有统计学意义(P<0.05).RA住院患者单核细胞PBK/TOPK表达低于门诊患者,且住院患者RF水平高于门诊患者,差异具有统计学意义(P<0.05).结论 单核细胞 PBK/TOPK低表达与疾病炎症有关,对类风湿关节炎的靶向治疗有一定意义.
Abstract
Objective To investigate the expression and clinical significance of PDZ-binding-kinase/T-LAK cell-derived protein kinase(PBK/TOPK)in monocytes of patients with rheumatoid arthritis(RA).Methods PBK/TOPK expression in peripheral blood mononuclear cells(PBMC)was detected by flow cytometry in 47 RA patients and 20 healthy subjects.Results The expression level of PBK/TOPK in peripheral blood CD14+monocytes in RA patients was higher than that in healthy subjects,and the difference was statistically significant(P<0.05).The proportion of CD14+monocytes in PBMC,white blood cell(WBC)count,rheumatoid factor(RF),immunoglobulin A(IgA)and immunoglobulin G(IgG)of CD14+monocytes in RA patients with low expression of PBK/TOPK were higher than those in RA patients with high expression of PBK/TOPK.The difference was statistically significant(P<0.05).The monocyte PBK/TOPK expression in inpatients with RA was lower than that in outpatients,and the RF level in inpatients was higher than that in outpatients.The difference was statistically significant(P<0.05).Conclusion The low expression of PBK/TOPK in monocytes is related to the inflammation of the disease,and it has certain significance for the targeted therapy of RA.
关键词
PBK/TOPK/类风湿关节炎/单核细胞Key words
PD2-binding-kinase/T-LAK cell-originated protein kinase/rheumatoid arthritis/monocyte引用本文复制引用
基金项目
国家自然科学基金地区科学基金(82360330)
广西自然科学基金面上项目(2020JJA140128)
广西脑与认知神经科学重点实验室开放课题(2020)(GKLBCN-20200108-06)
广西肿瘤免疫与微环境调控重点实验室开放基金(2202KF004)
广西医疗卫生重点培育学科建设项目()
出版年
2024
NETL
NSTL
万方数据