Prognostic value of miRNAs and their target genes in renal cell carcinoma
Objective Based on the cancer genome atlas(TCGA),the miRNAs prognostic risk scoring model of renal cancer was constructed to evaluate the potential clinical value of miRNAs and its target genes as molecular markers for renal cancer prognosis.Methods Based on TCGA miRNAs and mRNA datasets,the prognostic risk scoring model of renal cancer miRNAs was constructed and verified by single-factor,Lasso and multi-factor Cox regression analysis.GO and KEGG enrichment analyses of target genes were performed to predict the related functions of miRNAs in prognostic risk scoring model of renal cancer.The expressions of miRNAs and their target genes in the prognostic risk scoring model of renal cancer were verified in clinical samples.Results Based on the TCGA database,94 differential expression miRNAs and 1 226 mRNAs were identified in the transcriptome data of renal cancer samples compared with pericarcinomatous tissue.7 miRNAs were screened by independent prognostic analysis and a prognostic risk scoring model of renal cancer was constructed.GO and KEGG enrichment analyses showed that the functions of the 7 miRNAs were related to the PI3K-Akt signaling pathway,calcium ion signaling pathway and MAPK signaling pathway.The results of qPCR verification in renal cancer tissues samples showed that the expression patterns of 5 miRNAs and 8 target genes of miRNAs in clinical samples were consistent with the trend in TCGA database.Imunohistochemistry results showed that HPGD was low expressed in renal cancer.Conclusion The miRNAs prognostic model constructed in this study can effectively screen for miRNAs related to the prognosis of renal cancer.The study of these miRNAs is of great significance for the mechanism of renal cell carcinoma occurrence and development,as well as the prognosis of renal cell cancer.
renal cancermiRNAstarget geneprognostic risk scoring model
NETL
NSTL
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