山茶花内生真菌次生代谢产物化学成分及降糖活性分析
Chemical constituents and hypoglycemic activities analysis of secondary metabolites of the endophytic fungus from camellia sp.
冼小雅 1赵苗 1梁琳 1闫梦麒 1周先丽 2梁成钦1
作者信息
- 1. 桂林医学院 药学院,桂林 541199
- 2. 桂林医学院 智能医学与生物技术学院,桂林 541199
- 折叠
摘要
目的 探索山茶花内生真菌Paraconiothyrium brasiliense次生代谢产物化学成分及降糖作用.方法 使用硅胶柱色谱、薄层色谱(TLC)及高效液相色谱(HPLC)等分离纯化真菌发酵后的乙酸乙酯部位提取物,根据波谱方法与文献数据对比进行结构表征.采用pNPG法对分离得到的化合物进行降糖活性筛选,并用分子对接的手段进一步研究化合物对α-葡萄糖苷酶的抑制作用.结果 从真菌发酵后的乙酸乙酯部位提取物中分离得到11 个已知化合物,并检测了降糖活性.化合物 6 对α-葡萄糖苷酶有抑制活性,IC50为(15.48±11.82).分子对接表明,化合物 6 结合能为-6.5 kJ/mol.结论 化合物1～5、7～11 为首次从真菌Paraconiothyrium brasiliense中分离得到,其中化合物 6 对α-葡萄糖苷酶有较强的抑制作用.
Abstract
Objective To study the chemical composition of the endophytic fungus Paraconiothyrium brasiliense from camellia sp.and their hypoglycemic effects.Methods The ethyl acetate extracts were separated and purified by silica gel column chromatography,thin layer chromatography(TLC),and high performance liquid chromatography(HPLC).The structures of the obtained compounds were characterized according to spectroscopic methods or by comparison with relevant literature data.The hypoglycemic activity of the isolated compounds was screened by pNPG method,and the inhibitory mechanism of α-glucosidase was further studied by molecular docking method.Results Eleven known compounds were isolated from the ethyl acetate extract after fungal fermentation.The glucose-lowering activity assay indicated that compound 6 had an inhibitory activity on α-glucosidase with IC50 value of(15.48±11.82).Molecular docking assay showed that the binding energy of compound 6 was-6.5 kJ/mol.Conclusion Compounds 1～5,7～11 are isolated for the first time from the fungus Paraconiothyrium brasiliense.Compound 6 shows a moderate inhibitory effect on α-glucosidase.
关键词
山茶花内生真菌/次生代谢产物/降糖活性Key words
Paraconiothyrium Brasiliense/fungus/secondary metabolites/hypoglycemic activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据