华支睾吸虫成虫粗抗原及ESP促肝星状细胞活化效果比较
Comparison of effects between adult crude antigen and ESP on hepatic stellate cells activation
李佳 1蓝阳秋 1彭小红1
作者信息
- 1. 桂林医学院基础医学院,桂林 541199
- 折叠
摘要
目的 研究华支睾吸虫分泌排泄产物(Cs.ESP)及虫体粗抗原(Cs.CA)对小鼠原代肝星状细胞活化的影响.方法 分离小鼠原代肝星状细胞,鉴定后进行体外培养.实验分组为Cs.ESP刺激组和Cs.CA刺激组,同时设立空白对照组;采用 RT-PCR 检测肝星状细胞活化指标Ⅰ型胶原蛋白(CollagenⅠ)、α-平滑肌肌动蛋白(SMA)的mRNA转录水平,采用Western blot检测CollagenⅠ、α-SMA蛋白表达情况.结果 Cs.ESP 刺激组较Cs.CA刺激组和空白对照组的CollagenⅠ、α-SMA的mRNA转录水平及蛋白表达水平均上调,差异均有统计学意义(P<0.05),而Cs.CA刺激组与空白对照组比较,差异无统计学意义(P>0.05).结论 Cs.ESP 可促进体外培养的肝星状细胞活化,而Cs.CA对其活化无明显作用,为后续华支睾吸虫致肝纤维化的细胞分子机制研究提供参考.
Abstract
Objective To study the effect of excretory products of Clonorchis sinensis(Cs.ESP)and crude antigen of crude-antigen of Clonorchis sinensis(Cs.CA)on the activation of primary cultured hepatic stellate cells(HSCs)from mice.Methods The primary HSCs from mice were separated,identified and cultured in vitro.HSCs were divided into the Cs.ESP stimulation group,Cs.CA stimulation group,and a blank control group.The mRNA levels of Collagen I and α-smooth muscle actin(α-SMA),the activation indicators of HSCs were detected by RT-PCR,and the protein expressions of Collagen I and α-SMA were detected by Western blot.Results The mRNA and protein expression levels of CollagenⅠ and α-SMA in the Cs.ESP stimulation group were up-regulated compared with those in the Cs.CA stimulation group and blank control group,showing a statistically significant difference(P<0.05).There was no significant difference in the mRNA and protein expression levels of CollagenⅠand α-SMA between Cs.CA stimulation group and blank control group(P>0.05).Conclusion Cs.ESP promotes the activation of HSCs in vitro,while Cs.CA has no obvious effect on the activation of HSCs.This study provides a reference for further research on the cellular and molecular mechanisms of liver fibrosis induced by Cs.ESP.
关键词
华支睾吸虫/排泄分泌产物/虫体粗抗原/肝星状细胞Key words
Clonorchis sinensis/excrete secretory products/crude-antigen of Clonorchis sinensis/hepatic stellate cells引用本文复制引用
基金项目
国家自然科学基金项目(82060376)
国家级大学生创新创业训练计划项目(202210601001)
出版年
2024
NETL
NSTL
万方数据