Comparison of effects between adult crude antigen and ESP on hepatic stellate cells activation
Objective To study the effect of excretory products of Clonorchis sinensis(Cs.ESP)and crude antigen of crude-antigen of Clonorchis sinensis(Cs.CA)on the activation of primary cultured hepatic stellate cells(HSCs)from mice.Methods The primary HSCs from mice were separated,identified and cultured in vitro.HSCs were divided into the Cs.ESP stimulation group,Cs.CA stimulation group,and a blank control group.The mRNA levels of Collagen I and α-smooth muscle actin(α-SMA),the activation indicators of HSCs were detected by RT-PCR,and the protein expressions of Collagen I and α-SMA were detected by Western blot.Results The mRNA and protein expression levels of CollagenⅠ and α-SMA in the Cs.ESP stimulation group were up-regulated compared with those in the Cs.CA stimulation group and blank control group,showing a statistically significant difference(P<0.05).There was no significant difference in the mRNA and protein expression levels of CollagenⅠand α-SMA between Cs.CA stimulation group and blank control group(P>0.05).Conclusion Cs.ESP promotes the activation of HSCs in vitro,while Cs.CA has no obvious effect on the activation of HSCs.This study provides a reference for further research on the cellular and molecular mechanisms of liver fibrosis induced by Cs.ESP.
Clonorchis sinensisexcrete secretory productscrude-antigen of Clonorchis sinensishepatic stellate cells
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