首页|广西"桂十味"药材体外抗氧化活性及保护作用

广西"桂十味"药材体外抗氧化活性及保护作用

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目的 探讨广西"桂十味"药材不同溶剂提取物体外抗氧化活性及对H2O2 诱导HepG2 和L02 细胞氧化损伤的保护作用。方法 以广西"桂十味"各味药材为原料，利用水和 75％乙醇制备不同溶剂提取物，通过DPPH、ABTS自由基清除法和Fe3+还原能力法评价其抗氧化活性，采用MTT法评价提取物对H2O2 诱导细胞氧化损伤的保护作用。结果 鸡血藤水提取物表现出最强的DPPH和ABTS自由基清除能力，鸡血藤 75％乙醇提取物表现出最强的Fe3+还原能力;山豆根水提取物及鸡血藤75％乙醇提取物对H2O2 诱导HepG2 细胞氧化损伤的保护作用最强;鸡骨草水提取物及龙眼肉、莪术 75％乙醇提取物对H2O2 诱导L02 细胞氧化损伤的保护作用最强。结论 通过比较"桂十味"各味药材不同溶剂提取物的抗氧化活性及对H2O2 诱导细胞氧化损伤的保护作用，可为后期"桂十味"道地药材的抗氧化研发提供实验基础。
Antioxidant activity and protective effect of ten varieties geo-authentic traditional Chinese medicine of Guangxi
This article aims to investigate the antioxidant activity in vitro and protective effect against H2O2-induced oxidative damage of different solvent extracts of"ten varieties geo-authentic traditional Chinese medicine of Guangxi"in HepG2 and L02 cells.Methods With"ten varieties geo-authentic traditional Chinese medicine of Guangxi"as the raw materials,their different solvent extracts were prepared with water and 75％ethanol.The antioxidant activities of different solvent extracts were evaluated by DPPH,ABTS radical scavenging method and Fe3+reduction capacity method.MTT method was used to evaluate the protective effect of different solvent extracts on H2O2 induced cells oxidative damage.Results The aqueous extract of Spatholobus suberectus Dunn exhibited the strongest scavenging ability for DPPH and ABTS free radicals.The 75％ethanol extract of Spatholobus suberectus Dunn showed the strongest reducing ability for Fe3+.The aqueous extracts of Euchresta japonica Hook.f.ex Rege and 75％ethanol extract of Cassia Twig and Spatholobus suberectus Dunn had the strongest protective effect on the cell viability of HepG2 cells.The aqueous extracts of Abrus pulchellus subsp cantoniensis(Hance)Verdcourt and the 75％ethanol extract of Dimocarpus longan Lour and Curcuma kwangsiensis S.G.Lee et C.F.Liang had the strongest protective effect on the cell viability of L02 cells.Conclusion The antioxidant activity and the protective effect on H2O2-induced cellular oxidative damage of different solvent extracts of"ten varieties geo-authentic traditional Chinese medicine of Guangxi"are compared in this study,which provides an experimental basis for the later antioxidant research and development of"ten varieties geo-authentic traditional Chinese medicine of Guangxi".

ten varieties geo-authentic traditional Chinese medicine of Guangxidifferent solvent extractsantioxidant activityanti-cellular oxidative damage

张兵、陈聪、彭彦芬、韦坤华、余启明、谭相端

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桂林医学院 药学院,桂林 541199

桂林医学院 广西药物分子发现与成药性优化重点实验室,桂林 541199

桂林医学院 公共卫生学院,桂林 541199

广西药用植物园广西药用资源保护与遗传改良重点实验室,南宁 530023

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桂十味 不同溶剂提取物 抗氧化活性 抗细胞氧化损伤

国家自然科学基金项目广西自然科学基金项目广西研究生教育创新计划项目

820606272020GXNSFAA159149YCSW2024451

2024

10.19296/j.cnki.1008-2409.2024-04-002

华夏医学
桂林医学院

华夏医学

影响因子：0.569
ISSN：1008-2409
年,卷(期)：2024.37(4)