miRNA-146a protects skin microenvironment through apoptosis inhibition and extracellular matrix deposition
Objective To study the role and mechanism of miRNA-146a in skin repair.Methods The role of miRNA-146a in skin repair was evaluated by a skin injury model in zebra fish.Fibroblasts were treated with miRNA-146a mimic and the expression of extracellular matrix related genes were detected.The mRNA expression in keratinocytes treated with miRNA-146a mimic were analyzed by RNA deep sequencing.The target genes of miRNA-146a were predicted by miRWalk,miRBD and Targetscan databases.Results miRNA-146a mimic significantly reduced melanin deposition and promoted wound healing in damaged skin.RNA deep sequencing showed that miRNA-146a mimic protected against keratinocyte apoptosis.In addition,miRNA-146a mimic significantly promoted the extracellular matrix deposition in fibroblasts.Conclusion miRNA-146a promotes skin repair by inhibiting keratinocyte apoptosis and promoting extracellular matrix deposition in skin fibroblasts.This study provides new theoretical guidance for skin repair in clinical practice.
万方数据
维普
