Homology analysis of blaADC genes in cephalosporin-resistant Acinetobacter baumannii
Objective To analyze the homology of blaADC genes in multidrug-resistant Acinetobacter baumannii(MDRAB)strains resistant to cephalosporin antibiotics.Methods The bacterium isolated from clinical specimens was identified and antibiotic susceptibility testing was conducted by VITEK Auto Microbe System.The PCR amplification and unidirectional sequencing of blaADC gene amplification products were done.The Aligned sequences were compared with the GenBank database respectively and the homology was analyzed using MEGA 5.0 software in resistant to cephalosporin MDRAB strains.Results The blaADC genes were detected in all of 14 MDRAB strains and located on the bacterial chromosome,and their sequences were highly similar to those in the GenBank database.There was a high degree of homology among blaADC genes in the 12 MDRAB strains,indicating the possibility of horizontal transmission of cloned strains.Conclusion The resistance to cephalosporins in MDRAB strains is directly related to the blaADC gene carried by bacteria.Horizontal transmission of cloned strains can cause nosocomial infection,therefore,it is great significance for preventing nosocomial infection to take effective measures to control the spread of cloned resistant strains.
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