华夏医学2024，Vol.37Issue(6) ：51-57.DOI:10.19296/j.cnki.1008-2409.2024-06-007

miR-196a-5p靶向抑制HMGA1参与卵巢癌细胞上皮间质转化的机制

Mechanism of miR-196a-5p targeting the inhibition of HMGA1 involved in the epithelial mesenchymal transition of ovarian cancer cells

彭广涛 蒋晖 桂莎莎 唐娟 李秋萍
华夏医学2024，Vol.37Issue(6) ：51-57.DOI:10.19296/j.cnki.1008-2409.2024-06-007
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miR-196a-5p靶向抑制HMGA1参与卵巢癌细胞上皮间质转化的机制

Mechanism of miR-196a-5p targeting the inhibition of HMGA1 involved in the epithelial mesenchymal transition of ovarian cancer cells

彭广涛 1蒋晖 1桂莎莎 1唐娟 1李秋萍1
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作者信息

  • 1. 桂林市人民医院,桂林 541002
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摘要

目的 分析miR-196a-5p靶向抑制HMGA1 参与卵巢癌细胞上皮间质转化的机制.方法 收集桂林市人民医院病理科留取的卵巢癌组织、癌旁组织 50 例,并购买卵巢上皮细胞HOSEpiC、卵巢癌细胞系 A2780、SKOV3,检测miR-196a-5p、HMGA1 mRNA表达量.细胞培养完成后进行转染,48 h后检测各组细胞迁移、侵袭情况及上皮间质转化相关蛋白相对表达量.结果 与癌旁组织相比,癌组织中的 miR-196a-5p、HMGA1 mRNA表达量较高,差异有统计学意义(P<0.05),卵巢癌细胞系SKOV3 miR-196a-5p、HMGA1 mRNA表达量最高,故选择 SKOV3 细胞进行后续实验.与 miR-196a-5p-NC 组相比,si-miR-196a-5p 组 miR-196a-5p、Vimentin、Snail表达量、细胞迁移数及侵袭数下降,E-cadherin表达量上升(P<0.05).与HMGA1-NC组相比,si-HMGA1 组HMGA1、Vimentin、Snail表达量、细胞迁移数及侵袭数下降,E-cadherin表达量上升(P<0.05).与HMGA1-NC组相比,HMGA1 组野生型WT-miR-196a-5p的荧火虫荧光素酶相对活性较高(P<0.05),而突变型MUT-miR-196a-5p 无显著变化(P>0.05).与 si-miR-196a-5p+HMGA1-NC 组相比,si-miR-196a-5p+HMGA1 组HMGA1、Vimentin、Snail 表达量、细胞迁移数及侵袭数上升,E-cadherin 表达量下降(P<0.05).结论 下调miR-196a-5p通过靶向抑制HMGA1 而控制细胞迁移及侵袭,抑制上皮间质转化.

Abstract

Objective To analyze the mechanism of miR-196a-5p targeting the inhibition of HMGA1 involved in the epithelial mesenchymal transformation of ovarian cancer cells.Methods 50 cases of ovarian cancer tissues and adjacent tissues from the pathology department of our hospital were collected,and ovarian epithelial cells HOSEpiC cells,ovarian cancer cell line A2780 and SKOV3 cells were purchased.The levels of miR-196a-5p and mRNA expression of HMGA1 in cells were detected.After cell culture,the cells were transfected.After transfection for 48 h,the migration and invasion of cells and the relative expression of proteins related to epithelial mesenchymal transformation were detected in each group.Results The levels of miR-196a-5p and mRNA expression of HMGA1 in cancer tissues were significantly higher than those in adjacent tissues,showing a statistically significomt difference(P<0.05).The levels of miR-196a-5p and HMGA1 mRNA expression in ovarian cancer cell line SKOV3 were the highest,so SKOV3 cells were selected for follow-up experiments.Compared with miR-196A-5P-NC group,the expression levels of miR-196a-5p,Vimentin and Snail,cell migration and invasion were significantly decreased in si-miR-196a-5p group,while the expression level of E-cadherin was significantly increased(P<0.05).Compared with the HMGA1-NC group,the expressions of HMGA1,Vimentin and Snail,the cell migration and invasion were significantly decreased in the si-HMGA1 group,while the expression of E-cadherin was significantly increased(P<0.05).Compared with the HMGA1-NC group,the relative fluorescein activity of wild-type WT-miR-196a-5p was significantly higher in the HMGA1 group(P<0.05),while the mutant MUT-miR-196a-5p had no significant change(P>0.05).Compared with the si-miR-196a-5p+HMGA1-NC group,the expressions of HMGA1,Vimentin and Snail,cell migration and invasion were significantly increased in the si-miR-196a-5p+HMGA1 group,while the expression of E-cadherin was significantly decreased(P<0.05).Conclusion Down-regulated miR-196a-5p controls the cell migration and invasion and inhibits the epithelial mesenchymal transformation by targeting the HMGA1 inhibition.

关键词

微小RNA-196a-5p/高迁移率族蛋白组A1/卵巢癌/上皮间质转化

Key words

micR-196a-5p/high mobility group proteome A1/ovarian cancer/epithelial mesenchymal transformation

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出版年

2024
华夏医学
桂林医学院

华夏医学

影响因子：0.569
ISSN：1008-2409
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