Mechanism of miR-196a-5p targeting the inhibition of HMGA1 involved in the epithelial mesenchymal transition of ovarian cancer cells
Objective To analyze the mechanism of miR-196a-5p targeting the inhibition of HMGA1 involved in the epithelial mesenchymal transformation of ovarian cancer cells.Methods 50 cases of ovarian cancer tissues and adjacent tissues from the pathology department of our hospital were collected,and ovarian epithelial cells HOSEpiC cells,ovarian cancer cell line A2780 and SKOV3 cells were purchased.The levels of miR-196a-5p and mRNA expression of HMGA1 in cells were detected.After cell culture,the cells were transfected.After transfection for 48 h,the migration and invasion of cells and the relative expression of proteins related to epithelial mesenchymal transformation were detected in each group.Results The levels of miR-196a-5p and mRNA expression of HMGA1 in cancer tissues were significantly higher than those in adjacent tissues,showing a statistically significomt difference(P<0.05).The levels of miR-196a-5p and HMGA1 mRNA expression in ovarian cancer cell line SKOV3 were the highest,so SKOV3 cells were selected for follow-up experiments.Compared with miR-196A-5P-NC group,the expression levels of miR-196a-5p,Vimentin and Snail,cell migration and invasion were significantly decreased in si-miR-196a-5p group,while the expression level of E-cadherin was significantly increased(P<0.05).Compared with the HMGA1-NC group,the expressions of HMGA1,Vimentin and Snail,the cell migration and invasion were significantly decreased in the si-HMGA1 group,while the expression of E-cadherin was significantly increased(P<0.05).Compared with the HMGA1-NC group,the relative fluorescein activity of wild-type WT-miR-196a-5p was significantly higher in the HMGA1 group(P<0.05),while the mutant MUT-miR-196a-5p had no significant change(P>0.05).Compared with the si-miR-196a-5p+HMGA1-NC group,the expressions of HMGA1,Vimentin and Snail,cell migration and invasion were significantly increased in the si-miR-196a-5p+HMGA1 group,while the expression of E-cadherin was significantly decreased(P<0.05).Conclusion Down-regulated miR-196a-5p controls the cell migration and invasion and inhibits the epithelial mesenchymal transformation by targeting the HMGA1 inhibition.
micR-196a-5phigh mobility group proteome A1ovarian cancerepithelial mesenchymal transformation
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