Antibiotic resistant genes in aquacultural bacteria
54 aquacultural bacterial strains originated from different area were tested for the drug sensitivity of 10 common antibiotics determined by Kirby-Bauer disk diffusion according to NCCLS. With specific primers designe according to the reported sequences in GenBank, polymerase chain reaction (PCR) assay were used to amplify the antibiotic resistant genes and study the mechanisms of drug resistance. 42 of 54 gram-negative bacteria were identified as resistant bacteria (77. 8%), and 37 strains (68. 5% ) had the multi-resistances for resistance to more than 3 antibiotics. The resistant genes on plasraids were detected by PCR method. 9 strains were tested including trimethoprim-sulfamethoxazole resistance gene sul2, 3 strains possessed chloramphenicol resistant gene cat2, 4 strains have chloramphenicol resistant gene cat3, only 1 strain was amplified to have chloramphenicol resistant gene cat4, and 3 strains were detected with kanamyein resistant gene sadB. The detection ratios of the resistant genes in plasmid were 50% , 27% , 36% , 9% , 60% , respectively.
NETL
NSTL
万方数据
维普
