中肋骨条藻快速可视化现场检测方法的建立与应用
Establishment and application of rapid visual in-situ detection method for Skeletonema costatum
杨超 1甄毓 2秦铭俐 3朱吉炜1
作者信息
- 1. 中国海洋大学环境科学与工程学院海洋环境与生态教育部重点实验室,山东青岛 266100
- 2. 中国海洋大学环境科学与工程学院海洋环境与生态教育部重点实验室,山东青岛 266100;青岛海洋科学技术国家实验室海洋生态与环境科学功能实验室,山东青岛 266071
- 3. 自然资源部宁波海洋中心,浙江宁波 315012
- 折叠
摘要
中肋骨条藻(Skeletonema costatum)是一种全球海域广布种与赤潮多发种,对其进行检测与监测对海洋生态环境研究具有重要意义.本研究以核酸快速提取方法及环介导恒温扩增技术为基础,建立了一种针对中肋骨条藻的快速可视化现场检测方法.该方法特异性强、灵敏度高(可达 1.2 pg基因组DNA)、检测时间短(100 min以内),可通过反应体系颜色变化对藻细胞有无进行快速判断.该方法经过大量野外样品的验证,具有较高的可行性,可被推广应用于中肋骨条藻的现场监测工作中.
Abstract
Skeletonema costatum is a widely distributed species in global waters,which also causes red tide frequently.It is of great significance to detect and monitor S.costatum for marine ecological environment research.In this study,a rapid visual in-situ detection method for S.costatum was developed based on rapid nucleic acid extraction and loop-mediated isothermal amplification.This method offers the advantages of high specificity,high sensitivity(1.2 pg genomic DNA)and rapid detection time(＜100 min),and the presence or absence of cells can be determined through color changes in the reaction system.The method has been verified by a large number of field samples and holds great feasibility,which is expected to be widely used in field monitoring of S.costatum.
关键词
赤潮/中肋骨条藻/现场检测/环介导恒温扩增Key words
red tide/Skeletonema costatum/in-situ detection/loop-mediated isothermal amplification引用本文复制引用
基金项目
国家重点研发计划课题(2022YFC3105202)
崂山实验室科技创新项目(LSKJ202203700)
宁波市自然资源和规划局项目(甬资规采合2023第56号)
出版年
2024
NETL
NSTL
万方数据