Establishment and application of rapid visual in-situ detection method for Skeletonema costatum
Skeletonema costatum is a widely distributed species in global waters,which also causes red tide frequently.It is of great significance to detect and monitor S.costatum for marine ecological environment research.In this study,a rapid visual in-situ detection method for S.costatum was developed based on rapid nucleic acid extraction and loop-mediated isothermal amplification.This method offers the advantages of high specificity,high sensitivity(1.2 pg genomic DNA)and rapid detection time(＜100 min),and the presence or absence of cells can be determined through color changes in the reaction system.The method has been verified by a large number of field samples and holds great feasibility,which is expected to be widely used in field monitoring of S.costatum.
red tideSkeletonema costatumin-situ detectionloop-mediated isothermal amplification
NETL
NSTL
万方数据
