Study on in vitro preservation of spermatozoa of Exopalae-mon carinicauda
In this paper, the effect of different diluting solutions and cryoprotectants on in vitro cryopreservation of spermatozoa was studied by evaluation of spermatozoa quality using survival rate and acrosin activity assays. The results showed that Ringer’s extender was the best diluting solution of spermatozoa in vitro preservation. After a preservation for 4 d at 4℃ with Ringer’s extender, the survival rate was 71.50%, and the acrosin activity reduced to 1.803μIU/106. Both of these two values were higher than those of other experimental groups. The survival rate was significantly correlated with acrosin activity in all the experimental groups, R=0.846 (P<0.05). After a preserva-tion for 2 d in liquid nitrogen with Ringer’s extender, the survival rate was 83.50%and the acrosin activity reduced to 2.507μIU/106. Similarly, both of them were higher than other groups, as well as the values obtained at 4℃. There existed a highly significant correlation between survival rate and acrosin activity, R=0.862 (P<0.01). 15%DMSO was the best cryoprotectant for cryopreservation. After a 2 d preservation, the survival rate was 81.32%, and the acrosin activity reduced to 1.385μIU/106. There existed a highly significant correlation between survival rate and acrosin activity, R=0.864 (P<0.01).
国家科技期刊平台
NSTL
万方数据
维普
