Study on extract and enzymolysis technology of protein from discarded fish bone
Different extraction methods and different protease digestion of discarded fish bone proteins were studied using extraction rate of proteins and molecular weight of peptides as evaluation indexes. The results showed that the method of high-pressure cooking had a significantly higher protein content of 86.15% and protein extraction rate of 16.76% than the methods of constant temperature water bath and hot reflux extraction. The neutral protease had a higher activity than other three kinds of proteases. The protein content could reach 88.46% and the peptide ratio could reach 95% in the protein hydrolysates using neutral protease. The enzyme dosage could be reduced when the protein was extracted with high-pressure cooking method and the polypeptide was prepared with neutral protease. This method could provide reference and new idea for polypeptide industrial production.
国家科技期刊平台
NSTL
万方数据
维普
