Microsatellite analysis of genetic diversity in five geographic populations of Tegillarca granosa
Genetic diversity was analyzed in five geographic populations of Tegillarca granosa by using 17 poly-morphic microsatellite markers. The five different populations were sampled in Wenzhou City, Zhejiang Province;Rizhao City, Shandong Province; Qisha City, Guangxi Province; Haikou City, Hannan Province and Korea. In the seventeen microsatellite markers, 115 alleles were found and allele number per locus was 2-12. Mean effective al-lele number , mean observed and expected heterozygosity, and mean polymorphism information content were 1.192-7.849, 0.430-0.516 and 0.573-0.656 and 0.525-0.608, respectively. The Hardy-weinberg balance test showed that 51.2% microsatellite sites deviated from equilibrium(P< 0.05). The FST value of the five geographic popula-tions ranged from 0.012 to 0.062, revealing a low level of genetic differentiation among them. The UPMGA tree indicated that Zhejiang and Korea populations clustered firstly, then clustered with Hainan population, and the Guangxi population clustered with Shandong population into another clade.