Effects of low temperature stress on heat shock and antifreeze proteins in Manila clam(Ruditapes philippinarum)
Manila clam(Ruditapes philippinarum)is one of the most commercially important aquaculture species in Asia.Three populations of 100 clams were exposed to acute low temperature(-1 ℃)stress for 9 days,and the sur-vival rates,in descending order,were the Zebra clam 2(78.08％±2.14％),the Dalian population(70.37％±3.70％),and the Beihai population(48.15％±7.71％).The expression of HSP70,HSC70,CSDE1,Y-box,and AFPⅡ genes was investigated in these populations(zebra clam 2,Dalian population,and Beihai population)under acute low temperature stress(-1 ℃).The results showed that the temporal expression of the five genes in the gill tissue of the Manila clam was significantly correlated with temperature(P＜0.05),among which HSP70 and HSC70 were sig-nificantly higher in the three populations(P＜0.05).The CSDE1 and Y-box genes of the antifreeze protein family were only highly expressed in the gill tissues of the Guangxi population treated with low temperatures.The relative expression of the AFP Ⅱ gene in the three populations increased significantly,reaching maximum values at highest at 12 h(P＜0.05).The relative gene expression was highest in Zebra Clam 2,followed by Zebra Clam 2 and the Beihai population(P＜0.05).The expression patterns of genes related to low temperature resistance were different in different populations of clams.The expression levels and survival rates of HSP70,HSC70,and AFPⅡ in Zebra Clam 2 were higher than those in the wild Dalian and Beihai populations.
Ruditapes philippinarumpopulationcold stresssurvival rateheat shock proteinantifreeze protein
万方数据
维普
