Manila clam(Ruditapes philippinarum)is one of the most commercially important aquaculture species in Asia.Three populations of 100 clams were exposed to acute low temperature(-1 ℃)stress for 9 days,and the sur-vival rates,in descending order,were the Zebra clam 2(78.08％±2.14％),the Dalian population(70.37％±3.70％),and the Beihai population(48.15％±7.71％).The expression of HSP70,HSC70,CSDE1,Y-box,and AFPⅡ genes was investigated in these populations(zebra clam 2,Dalian population,and Beihai population)under acute low temperature stress(-1 ℃).The results showed that the temporal expression of the five genes in the gill tissue of the Manila clam was significantly correlated with temperature(P＜0.05),among which HSP70 and HSC70 were sig-nificantly higher in the three populations(P＜0.05).The CSDE1 and Y-box genes of the antifreeze protein family were only highly expressed in the gill tissues of the Guangxi population treated with low temperatures.The relative expression of the AFP Ⅱ gene in the three populations increased significantly,reaching maximum values at highest at 12 h(P＜0.05).The relative gene expression was highest in Zebra Clam 2,followed by Zebra Clam 2 and the Beihai population(P＜0.05).The expression patterns of genes related to low temperature resistance were different in different populations of clams.The expression levels and survival rates of HSP70,HSC70,and AFPⅡ in Zebra Clam 2 were higher than those in the wild Dalian and Beihai populations.
关键词
菲律宾蛤仔/群体/低温/存活率/热休克蛋白/抗冻蛋白
Key words
Ruditapes philippinarum/population/cold stress/survival rate/heat shock protein/antifreeze protein
维普
万方数据