A comparison of the mitochondrial DNA-based genetic diver-sity of Kumamoto oyster populations from China,Japan,and the United States
A total of 188 Kumamoto oysters were collected from six wild populations in China(Nantong,Zhejiang,Fujian,Guangdong,and Guangxi),Japan,and the United States to study the genetic diversity of cultured and wild populations.The cytochrome oxidase C subunit I(COI)and mitochondrial noncoding region(MNR)of each oyster were amplified and sequenced,and their genetic diversity and structure were analyzed.The results showed that the wild Chinese population had greater genetic diversity and more haplotype diversity in MNR sequences,whereas the Japanese and American populations had relatively lesser genetic diversity.Regarding the COI sequence,the genetic differentiation coefficient(FST)among the five Chinese populations was lower(-0.015 57-0.001 65),while the FST between the Chinese populations and the American,Japanese populations was much higher,and the FST between the American and Japanese populations was also lower(0.008 22).The results of the MNR sequences analysis were consistent,but with a large and significant genetic differentiation between the American and Japanese populations(0.109 68),indicating the great potential of MNR sequences in population genetics.AMOVA and haplotype network maps revealed genetic variations between the Chinese,American,and Japanese populations.However,a certain kinship occurred between the American and Japanese populations,which was consistent with their introduction history.The wild Chinese Kumamoto oyster population exhibited broader genetic diversity,with no genetic differ-entiation among the five populations.In contrast,the wild Japanese population is at risk of germplasm resource decline.Additionally,the cultured American population also exhibited narrow genetic diversity due to multiple generations of artificial selection and aquaculture,with obvious genetic variations from the Chinese population.This study provides a reference for the subsequent selection of breeding populations and the combination of cross-breeding populations.
国家科技期刊平台
NSTL
万方数据
