Isolation,purification,and anticoagulant analysis of polysac-charides from sea cucumber Phyllophorus proteus
In this study,two sulfated polysaccharides were isolated from Phyllophorus proteus using enzymat-ic-alkaline coupling and Sepharose DEAE Fast Flow column chromatography,and Sephadex G100 column chro-matography was used for further purification.Physicochemical property analysis indicated that the two sea cucum-ber polysaccharides were fucan sulfate(FS)and fucosylated glycosaminoglycan(FG).Two low molecular weight polysaccharides—dFS and dFG—were obtained through the degradation of FS using 10 mmol/L TFA and degrada-tion of FG via the β-elimination method,respectively.The in vitro anticoagulant assay demonstrated that FG showed good anticoagulant activity and significantly prolonged the clotting times of activated partial thromboplas-tin time(APTT),prothrombin time(PT),thrombin time(TT),and FIB.dFG significantly prolonged the clotting times of APTT,TT,and FIB but showed no significant effect on the clotting time of PT.Therefore,adjusting the molecular weight of FG can enhance the selective effects on specific coagulation pathways,thus reducing the risk of bleeding while maintaining optimal anticoagulant activities.These results indicated that FG and dFG can be de-veloped as anticoagulants.
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