为了评估不同形态的球等鞭金藻(Isochrysis galbana)对菲律宾蛤(Ruditapes philippinarum)仔幼虫生长发育的效果,本研究选择人工培养的球等鞭金藻 3011、金藻粉和金藻泥作为饵料,投喂菲律宾蛤仔 D 形幼虫,测定了幼虫的密度、相对生长率以及淀粉酶(AMY)、丝裂原活化蛋白激酶 1(MAPK1)和胰岛素样生长因子(IGF)的基因表达水平.结果显示,投喂活体球等鞭金藻的菲律宾蛤仔幼虫在生长速度和存活率方面均优于投喂金藻粉和金藻泥的幼虫,表明干燥处理和浓缩冷冻会影响金藻的营养价值;投喂藻泥的蛤仔幼虫存活率高于藻粉组,但生长率低于藻粉组;饥饿的蛤仔幼虫生长速度、存活率、淀粉酶和生长发育相关基因的表达均处于较低水平.本研究表明,合适的饵料供给对菲律宾蛤仔幼虫的生长发育至关重要,金藻粉和金藻泥还不能完全替代活体金藻,在生产中可以作为添加剂部分替代活体金藻.本研究可为菲律宾蛤仔幼虫营养需求和工厂化人工育苗技术研究提供参考.
Effects of different forms of Isochrysis galbana on the growth and development of Ruditapes philippinarum larvae
The effect of different forms of Isochrysis galbana on the growth and development of D-shaped Ruditapes philippinarum larvae was evaluated by feeding them with artificially cultured I.galbana 3011,dried I.galbana powder,and concentrated liquid I.galbana.The larval density,relative growth rate,and expression levels of amylase(AMY),mitogen-activated protein kinase 1(MAPK1),and insulin-like growth factor(IGF)genes were determined.The results showed that larvae fed with live I.galbana outperformed those fed with I.galbana powder or concentrated liquid I.galbana in terms of growth rate and survival,indicating that drying and concentration freezing affected the nutritional value of I.galbana.The survival rate of larvae fed with concentrated liquid I.gal-bana was higher than that of larvae fed with algal powder,but the growth rate was lower.Decreased growth rate,survival,AMY,MAPK1,and IGF expression were observed in starved larvae.This study demonstrated that a correct food supply is essential for the growth and development of R.philippinarum larvae and that dried I.galbana pow-der or concentrated liquid I.galbana is not a complete substitute for live I.galbana and can be used as additives during production.This study provides a reference for the nutritional requirements of R.philippinarum larvae and large-scale artificial reproduction technology.
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