Distribution characteristics of blood cells in early zebrafish embryos
The zebrafish(Danio rerio)is a model organism for investigating animal developmental biology and is used to evaluate the mechanism and related pathological characteristics of vascular and blood diseases.Fertilized eggs of zebrafish were collected and incubated until hatching to determine blood circulation in early embryos.Quantitative polymerase chain reaction(PCR)was used to analyze the expression of genes related to blood cells,blood vessels,and hemoglobin at various developmental stages.Simultaneously,o-dianisidine was used to stain embryonic hemoglobin.Following staining,an anatomical lens and microscope were used to section,examine,and photograph the embryos.Gene expression data were compared with hemoglobin distribution to analyze the devel-opment of the blood system at different stages in early zebrafish embryos.Quantitative PCR results revealed that primitive hematopoiesis began at 12 h post-fertilization,blood vessel formation began at 24 h post-fertilization,and the blood circulation system was nearly fully developed by 72 h post-fertilization.However,staining observations showed a delay;red blood cells were first detected in the yolk sac of zebrafish embryos at 30 h post-fertilization,circulating blood in the heart at 48 h post-fertilization,and red blood cells in the trunk and somites by sectioning.Red blood cells can also be found in the brain 72 h post-fertilization of embryos by sectioning.Blood circulation in zebrafish embryos matured at 96 h post-fertilization.This study details the development timeline and distribution characteristics of blood vessels and blood cells(red blood cells mainly)at gene and protein levels,providing a foundation for analyzing pathological and toxicological features of blood-related diseases using simple detection methods.
国家科技期刊平台
NSTL
万方数据
