Green preparation of chitin from shrimp shells by two-step fermentation using microbiological method
Shrimp shells are currently the most important source of chitin.An environmentally friendly and efficient two-step fermentation method,i.e.,demineralization(DM)and deproteinization(DP),has been employed to extract chitin from shrimp shells.In this study,an acid-producing strain(Lactiplantibacillus plantarum)and a prote-ase-producing strain(Pseudomonas aeruginosa)were isolated from different sources and used to extract chitin from shrimp shells using a two-step fermentation method under optimal conditions,followed by decolorization with 10%H2O2.The sequence of DP followed by DM demonstrated superior calcium and protein removal,achieving 96.92%±0.07%and 78.46%±1.29%,respectively.Fourier transform infrared spectroscopy,X-ray diffraction,and scanning electron microscopy were used to characterize the product,all of which indicated a high degree of structural simi-larity to commercial chitin.The crystallization index was 78.70%,and the microstructure revealed a fibrous struc-ture with pores.These results indicated that the two-step fermentation method yields chitin of higher purity.This study provides a valuable reference for the effective extraction of chitin from shrimp shells using microbiological methods.
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