利用微生物法两步发酵从虾壳中提取甲壳素的绿色制备工艺研究
Green preparation of chitin from shrimp shells by two-step fermentation using microbiological method
周忆 1杨皓月 2邢荣娥 2刘松 2于华华 2李克成 2李鹏程2
作者信息
- 1. 中国科学院海洋研究所 实验海洋生物学重点实验室,山东 青岛 266071;中国科学院大学,北京 100049
- 2. 中国科学院海洋研究所 实验海洋生物学重点实验室,山东 青岛 266071
- 折叠
摘要
虾壳是目前甲壳素的最主要来源.作为一种环保高效的方法,采用脱钙、脱蛋白 2 种菌株分别作用的两步发酵法已被应用于从虾壳中提取甲壳素.首先,本文从不同来源分离得到产酸菌株植物乳杆菌(Lactiplantibacillus plantarum)及产蛋白酶菌株铜绿假单胞菌(Pseudomonas aeruginosa),进一步优化发酵条件,在最佳条件下采用两步发酵提取虾壳中的甲壳素,进一步采用10%H2O2脱色,其中先脱蛋白后脱钙的顺序综合表现出的钙和蛋白质的去除率更高,所得脱钙率及脱蛋白率分别为(96.92±0.07)%及(78.46±1.29)%.采用傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)及扫描电子显微镜(SEM)对所得产物进行表征,发现其与商用甲壳素结构相似度高,结晶指数为78.70%,微观结构表现为纤维状并带有孔隙.以上结果表明,利用这 2种微生物进行两步发酵法可获得较高纯度的甲壳素.本研究为利用微生物法有效提取虾壳来源的甲壳素提供了参考.
Abstract
Shrimp shells are currently the most important source of chitin.An environmentally friendly and efficient two-step fermentation method,i.e.,demineralization(DM)and deproteinization(DP),has been employed to extract chitin from shrimp shells.In this study,an acid-producing strain(Lactiplantibacillus plantarum)and a prote-ase-producing strain(Pseudomonas aeruginosa)were isolated from different sources and used to extract chitin from shrimp shells using a two-step fermentation method under optimal conditions,followed by decolorization with 10%H2O2.The sequence of DP followed by DM demonstrated superior calcium and protein removal,achieving 96.92%±0.07%and 78.46%±1.29%,respectively.Fourier transform infrared spectroscopy,X-ray diffraction,and scanning electron microscopy were used to characterize the product,all of which indicated a high degree of structural simi-larity to commercial chitin.The crystallization index was 78.70%,and the microstructure revealed a fibrous struc-ture with pores.These results indicated that the two-step fermentation method yields chitin of higher purity.This study provides a valuable reference for the effective extraction of chitin from shrimp shells using microbiological methods.
关键词
Lactiplantibacillus/plantarum/Pseudomonas/aeruginosa/微生物法/甲壳素提取Key words
Lactiplantibacillus plantarum/Pseudomonas aeruginosa/microbiological method/chitin extraction引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据