松江鲈幼鱼性腺分化的组织学观察
Histological observation of gonadal sex differentiation in Trachidermus fasciatus
高玉倩 1夏苏东 1高燕 2高丽 3李茂哲 1蒋泽昊 1薛东秀 4JIANG Zehao5
作者信息
- 1. 天津市水产生态及养殖重点实验室,天津农学院,天津 300384
- 2. 天津市水产研究所,天津 300221
- 3. 山东省科学院海洋仪器仪表研究所,山东 青岛 266001
- 4. 中国科学院海洋研究所,山东 青岛 266071
- 5. Tianjin Key Laboratory of Aquatic Ecology and Aquaculture,Tianjin Agricultural University,Tianjin 300384,China
- 折叠
摘要
为探究松江鲈(Trachidermus fasciatus Heckel)性腺分化和发育的规律,以孵化后 10～120 日龄的松江鲈幼鱼为研究对象,对其性腺进行常规石蜡切片和H-E组织染色法观察.结果表明:20 日龄幼鱼原始性腺形成;40 日龄幼鱼出现卵巢腔的雏形,标志着卵巢解剖学分化的开始;45 日龄幼鱼出现初级卵母细胞,表明卵巢开始进行细胞学分化;60 日龄幼鱼卵巢开始进入Ⅱ期,卵母细胞基本进入第Ⅱ时相;65 日龄幼鱼精巢处于Ⅰ期,输精小管的出现表明精巢开始进行解剖学分化;70 日龄幼鱼形成囊状的精原细胞团,表明精巢开始细胞学上的分化;90日龄幼鱼精巢进入Ⅱ期,初级精母细胞形成,细胞排列成束状.松江鲈鱼早期性腺分化属于分化型雌雄异体型,卵母细胞为部分同步型发育,且对脂质的积累能力相对较强,精巢的发育晚于卵巢,且其性腺分化方向受生殖细胞数量的影响.研究结果明确了松江鲈早期性腺分化过程及性腺分化关键时间节点,为其性别决定和分化机制研究提供了重要基础资料,也将为松江鲈人工繁育和人工性别控制诱导敏感期等提供理论参考.
Abstract
To elucidate the processes of gonadal differentiation and development in Trachidermus fasciatus,larvae at 10-120 days post-hatching(dph)were examined using conventional paraffin sections and hematoxylin-eosin tissue staining.The results revealed several key milestones:primordial gonadal formation at 20 dph,appearance of the embryonic ovarian cavity at 40 dph,initiation of ovarian cytological differentiation marked by primary oocytes at 45 dph,entry into stage Ⅱ at 60 dph,and appearance of fissure seminiferous tubules at 65 dph indicating the onset of anatomical differentiation of the testis(stage Ⅰ).Furthermore,at 70 dph,the formation of a spermatogonium ves-icle signaled the cytological differentiation of spermatogonia.By 90 dph,the presence of stage Ⅱ spermatocytes,forming primary spermatocytes arranged in bundles,marked a significant stage in testicular development.T.fasci-atus exhibited heteromorphic differentiation into males and females.The oocyte development was partially syn-chronous,and the ability of lipid accumulation is relatively strong.The differentiation time of the testis was later than that of the ovaries,and the differentiation direction of the gonads was affected by the number of germ cells.T.fasciatus displayed distinct reproductive characteristics,providing valuable insights into its gonadal development and differentiation.These results clarified the gonadal differentiation process and key time nodes,provided im-portant foundational data for studying the sex determination mechanism,and also contributed theoretical reference for the artificial breeding and sex control induction sensitive periods in T.fasciatus.
关键词
松江鲈/性腺分化/精巢/卵巢/组织学观察Key words
Trachidermus fasciatus/Sex differentiation/Testes/Ovaries/Histological observation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据