首页|Spontaneous mutations and mutational responses to penicillin treatment in the bacterial pathogen Streptococcus pneumoniae D39

Spontaneous mutations and mutational responses to penicillin treatment in the bacterial pathogen Streptococcus pneumoniae D39

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Bacteria with functional DNA repair systems are expected to have low mutation rates due to strong natural selection for genomic stability.However,our study of the wild-type Streptococcus pneumoniae D39,a pathogen responsible for many common diseases,revealed a high spontaneous mutation rate of 0.02 per genome per cell division in mutation-accumulation(MA)lines.This rate is orders of magnitude higher than that of other non-mutator bacteria and is characterized by a high mutation bias in the A/T direction.The high mutation rate may have resulted from a reduction in the overall efficiency of selection,conferred by the tiny effective population size in nature.In line with this,S.pneumoniae D39 also exhibited the lowest DNA mismatch-repair(MMR)efficiency among bacteria.Treatment with the antibiotic penicillin did not elevate the mutation rate,as penicillin did not induce DNA damage and S.pneumoniae lacks a stress response pathway.Our findings suggested that the MA results are applicable to within-host scenarios and provide insights into pathogen evolution.

Neutral evolutionMutation spectrumAntibioticsDNA mismatch repairOxidative damage repair

Wanyue Jiang、Tongtong Lin、Jiao Pan、Caitlyn E.Rivera、Clayton Tincher、Yaohai Wang、Yu Zhang、Xiang Gao、Yan Wang、Ho-Ching T.Tsui、Malcolm E.Winkler、Michael Lynch、Hongan Long

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Institute of Evolution and Marine Biodiversity,KLMME,Ocean University of China,Qingdao 266003,China

Laboratory for Marine Biology and Biotechnology,Laoshan Laboratory,Qingdao 266237,China

Department of Biology,Indiana University,Bloomington,IN 47405,USA

School of Mathematics Science,Ocean University of China,Qingdao 266000,China

State Key Laboratory of Microbial Technology,Microbial Technology Institute,School of Life Science,Shandong University,Qingdao 266237,China

Biodesign Center for Mechanisms of Evolution,Arizona State University,Tempe,AZ 85281,USA

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2024

10.1007/s42995-024-00220-6

海洋生命科学与技术(英文)

海洋生命科学与技术(英文)

ISSN：
年,卷(期)：2024.6(2)