首页|Examination of wnt signaling mediated melanin transport and shell color formation in Pacific oyster(Crassostrea gigas)
Examination of wnt signaling mediated melanin transport and shell color formation in Pacific oyster(Crassostrea gigas)
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Mollusca exhibit remarkable diversity in shell coloration,attributed to the presence of melanin,a widely distributed pigment with various essential roles,such as mechanical strengthening,antioxidation and thermoregulation.However,the regulatory network governing melanogenesis and melanin transport in molluscs remains poorly understood.In this study,we conducted a systematic analysis of melanin distribution and transport in the Pacific oyster,utilizing light microscopy and high-resolution transmission electron microscopy.In addition,we characterized CgWnt1 and CgWnt2b-a in Crassostrea gigas,and analyzed Wnt signaling in melanocyte formation.Expression analysis revealed that these genes were predominantly expressed in the mantle of black-shelled individuals,particularly in the outer fold of the mantle.Furthermore,we employed RNA interference and inhibitors to specifically inhibit Wnt signaling in both in vivo and in vitro.The results revealed impaired melanogenesis and diminished tyrosinase activity upon Wnt signaling inhibition.These findings suggest the crucial role of Wnt ligands and downstream factors in melanogenesis.In summary,our study provides valuable insights into the regulatory mechanism of shell pigmentation in C.gigas.By demonstrating the promotion of melanogenesis through Wnt signaling modulation,we contribute to a better understanding of the complex processes underlying molluscan melanin production and shell coloration.
MelanogenesisMelanosome traffickingWIF-1
Yue Min、Qi Li、Hong Yu、Shaojun Du
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Key Laboratory of Mariculture,Ministry of Education,and College of Fisheries,Ocean University of China,Qingdao 266003,China
Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China
Institute of Marine and Environmental Technology,Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine,Baltimore 21240,USA
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