首页|The potent osteo-inductive capacity of bioinspired brown seaweed-derived carbohydrate nanofibrous three-dimensional scaffolds

The potent osteo-inductive capacity of bioinspired brown seaweed-derived carbohydrate nanofibrous three-dimensional scaffolds

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This study aimed to investigate the osteo-inductive capacity of a fucoidan polysaccharide network derived from brown algae on human adipose-derived stem cells(HA-MSCs)for bone regeneration.The physiochemical properties of the scaffold including surface morphology,surface chemistry,hydrophilicity,mechanical stiffness,and porosity were thoroughly charac-terized.Both in vitro and in vivo measurements implied a superior cell viability,proliferation,adhesion,and osteo-inductive performance of obtained scaffolds compared to using specific osteogenic induction medium with increased irregular growth of calcium crystallites,which mimic the structure of natural bones.That scaffold was highly biocompatible and suitable for cell cultures.Various examinations,such as quantification of mineralization,alkaline phosphatase,gene expression,and immunocytochemical staining of pre-osteocyte and bone markers confirmed that HAD-MSCs differentiate into osteoblasts,even without an osteogenic induction medium.This study provides evidence for the positive relationship and synergistic effects between the physical properties of the decellularized seaweed scaffold and the chemical composition of fucoidan in promoting the osteogenic differentiation of HA-MSCs.Altogether,the natural matrices derived from brown seaweed offers a sustainable,cost-effective,non-toxic bioinspired scaffold and holds promise for future clinical applications in orthopedics.

Bone tissue engineeringSeaweed-derived scaffoldFucoidansHA-MSCs differentiationOsteoblasts

Zahra Dadashi Ouranj、Saadi Hosseini、Atefeh Alipour、Shahin Homaeigohar、Shahram Azari、Leila Ghazizadeh、Mohammadali Shokrgozar、Sabu Thomas、Saeed Irian、Hosein Shahsavarani

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Department of Cell and Molecular Biology,Faculty of Biological Sciences,Kharazmi University,Tehran 15719-14911,Iran

Laboratory of Regenerative Medicine and Biomedical Innovations,National Cell Bank of Iran,Pasteur Institute of Iran,Tehran 13169-43551,Iran

Department of Nanobiotechnology,Pasteur Institute of Iran,Tehran 13169-43551,Iran

School of Science and Engineering,University of Dundee,Dundee DD1 4HN,UK

School of Chemical Sciences,Mahatma Gandhi University,Kottayam,Kerala,India

Department of Cell and Molecular Biology,Faculty of Life Sciences and Biotechnology,Shahid Beheshti University,Tehran 19839-69411,Iran

Iranian Biological Resource Center,Academic Center for Education,Culture and Research(ACECR),Tehran 1533734716,Iran

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2024

10.1007/s42995-024-00241-1

海洋生命科学与技术(英文)

海洋生命科学与技术(英文)

ISSN：
年,卷(期)：2024.6(3)