Exogenous indole modulates several CpxRA-mediated virulence-related parameters of Edwardsiella piscicida in vitro

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外文摘要：Indole signaling has been regarded as a promising target to control aquatic diseases.However,the relationship between exogenous indole and the virulence of Edwardsiella piscicida is obscure.E.piscicida is a facultative intracellular pathogen,and has been a model strain in aquaculture.In this study,we investigated the effect of exogenous indole on stress resistance and virulence of E.piscicida in the presence of and absence of endogenous indole(WT and ΔtnaA,respectively).Our results showed that exogenous indole reduced the resistance of WT against strong acidic stress,but enhanced that of ΔtnaA.Also,we found that exogenous indole abated viability of E.piscicida at high temperature,repressed bacterial biofilm formation,impaired bacterial envelope integrity,and weakened bacterial proliferation in macrophages,irrespective of the presence or absence of endogenous indole.These virulence-related phenotypes caused by exogenous indole are reasonably explained by the observation that exogenous indole downregulated the expressions of CpxRA and its target YccA via being responded by CpxA.The effects of exogenous indole on strong acid resistance are partially achieved by changing the expression of GadD,the key functional enzyme of acid resistance system(AR2).We believe that this is the first report about the impact of exogenous indole on strong acid stress and membrane integrity of pathogenic bacteria.Also,we reveal the likely mechanism by which exogenous indole regulates the expressions of virulence-related genes.These findings provide a new understanding on pathogenesis of E.piscicida and contribute to the prevention and control strategies of edwardsiellosis.

外文关键词：

Edwardsiella piscicidaIndoleCpxRAStress resistanceVirulence

作者：

Yin Gou、Qingjian Fang、Hanjie Gu、Aijun Tian、Jun Li、Yan Wang、Yonghua Hu

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作者单位：

National Key Laboratory for Tropical Crop Breeding,Zhanjiang Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,China

Key Laboratory of Biology and Genetic Resources of Tropical Crops,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China

School of Marine Sciences,Ningbo University,Ningbo 315211,China

Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources,Haikou 571101,China

School of Science and Medicine,Lake Superior State University,Sault Ste.Marie,MI 49783,USA

Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences,Sanya 572025,China

Laboratory for Marine Biology and Biotechnology,Qingdao Marine Science and Technology Center,Qingdao 266071,China

Sanya Research Institute of Chinese Academy of Tropical Agri

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出版年：

2024

DOI：

10.1007/s42995-024-00238-w

海洋生命科学与技术(英文)

ISSN：

年,卷(期)：2024.6(4)