The typical open vascular mollusk,the Pacific oyster(Crassostrea gigas),exhibits a behavior of opening and closing its shell,which easily allows a large amount of seawater to enter its body.As a result,microorganisms in the seawater readily attach to the soft tissue surfaces of the C.gigas.In this study,microbial diversity comparisons were conducted using 16S rRNA analysis between the colored and colorless areas of the C.gigasmantle tissues as well as the surrounding seawater in its habitat.The results revealed that both microbial diversity and richness on the surface of the C.gigasmantles were higher than in the seawater.At the phylum level,Proteobacteria was a common dominant phylum on both the colored and colorless areas of the mantles,whereas the dominant phylum in the seawater was Bacteroidetes.At the genus level,28 bacterial genera were identified,including Vibrio,Pseudoalteromonas,and Marinobacter.Furthermore,Linear Discriminant Analysis indicated the presence of microbial biomarker differences between the colored and colorless areas of the mantles.The primary biomarker for the colored area was norank_f_Spirochaetaceae,while for the colorless area,Vibrio was the major biomarker.In conclusion,significant differences exist in the microbial composition between the surface of the C.gigasmantle tissues and the surrounding seawater.Despite the colored and colorless areas originating from the same tissue of the mantles,they still exhibited differences in several microbial biomarkers.This study elucidates the variations in microbial communities among the colored and colorless areas of the mantles as well as the seawater,laying the foundation for future investigations into the mechanisms underlying interactions between different colored tissues and microorganisms.
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