Enhancement of eicosapentaenoic acid content by NoFAD1001-overexpression in Nannochloropsis oceanica
As an essential omega-3 fatty acid,eicosapentaenoic acid(EPA)has been shown to be effective in weight loss,anti-inflammatory and cardiovascular disease prevention.Nannochloropsis oceanica,which contains rich EPA,has been shown to have the potential to become an EPA cell factory due to its fast growth and high stress tolerance,its potential for large-scale cultivation,and its mature genetic manipulation system.Desaturase plays a key role in EPA synthesis,and overexpression of desaturase has been shown to increase the omega-3 fatty acid content of Chlamydomonas reinhardtii and Phaeodactylum tricornutum.However,the function and application of desaturase in N.oceanica have not been sufficiently studied.In order to enhance the EPA yield in N.oceanica,a desaturase-coding gene NoFAD1001 was isolated from N.oceanica genome.The silico results showed that the amino acid sequence of NoFAD1001 possessed 39%similarity to the counterpart of Mortierella alpina.The total EPA content of NoFAD1001-overexpression was increased by 22%-49%in N.oceanica,compared with that of wild type(WT).The triacylglycerol-associated EPA content of NoFAD1001-overexpression was also increased EPA content by 36%-73%.Meanwhile,the growth indexes of the overexpression strain were not significantly different from those of the wild type,indicating that the growth of the overexpression strain was not affected by the overexpression of NoFAD1001.By overexpression of NoFAD1001 in N.oceanica,this study provides a reference for the analysis of the molecular breeding and metabolism mechanism of omega-3 fatty acids in marine microalgae.
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