首页|Thiosulfate oxidation and autotrophy potential by marine pre-valent heterotrophic bacteria of genus Marinobacter

Thiosulfate oxidation and autotrophy potential by marine pre-valent heterotrophic bacteria of genus Marinobacter

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The genus Marinobacter is very broadly distributed in global environments and is considered as aerobic heterotroph.In this study,six Marinobacter strains were identified with autotrophic thiosulfate oxidation capacity.These strains,namely Marinobacter guineae M3BT,Marinobacter aromaticivorans D15-8PT,Marinobacter vulgaris F01T,Marinobacter profundi PWS21T,Marinobacter denitrificans JB02H27T,and Marinobacter sp.ST-1M(with a 99.93%similarity to the 16S rDNA sequences of Marinobacter salsuginis SD-14BT),were screened out of 32 Marinobacter strains by autotrophic thiosulfate oxidization medium.The population of cells grew in a chemoli-thotrophic medium,increasing from 105 cells/mL to 107 cells/mL within 5 d.This growth was accompanied by the consumption of thiosulfate 3.59 mmol/L to 9.64 mmol/L and the accumulation of sulfate up to 0.96 mmol/L,and occasionally produced sulfur containing complex particles.Among these Marinobacter strains,it was also found their capability of oxidizing thiosulfate to sulfate in a heterotrophic medium.Notably,M.vulgaris F01T and M.antarcticus ZS2-30T showed highly significant production of sulfate at 9.45 mmol/L and 3.10 mmol/L.Genome annotation indicated that these Marinobacter strains possess a complete Sox cluster for thiosulfate oxidation.Further phylogenetic analysis of the sox B gene revealed that six Marinobacter strains formed a separate lineage within Gammaproteobacteria and close to obligate chemolithoautotroph Thiomicrorhabdus arctica.The results indicated that thiosulfate oxidizing and chemolithoautotrophic potential in Marinobacter genus,which may contribute to the widespread of Marinobacter in the global ocean.

Marinobacterthiosulfate oxidationautotrophicmixotroph

Fei Xu、Xiang Zeng、Yadong Gong、Zongze Shao

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School of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China

Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China

Faculty of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,China

Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen,361005,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key R&D Program of ChinaScientific Research Foundation of Third Institute of Oceanography,MNR

91951201420304122021YFF05013042019021

2024

海洋学报(英文版)
中国海洋学会

海洋学报(英文版)

CSTPCD
影响因子:0.323
ISSN:0253-505X
年,卷(期):2024.43(8)