首页|Morphological,phylogenetic and metabolite profile of Proro-centrum clipeus,a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

Morphological,phylogenetic and metabolite profile of Proro-centrum clipeus,a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

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More than 30 species of benthic Prorocentrum have been identified,some of which produce okadaic acid(OA)and its derivatives,dinophysistoxins(DTXs),which cause diarrhetic shellfish poisoning(DSP).Increasing numbers of benthic Prorocentrum species have been reported in tropical and subtropical waters of China.In contrast,only a few benthic Prorocentrum species have been reported in temperate waters.In this study,morphological descriptions obtained using light microscopy,scanning electron microscopy and molecular characterization of one Prorocentrum clipeus strain isolated from the Yellow Sea are presented.Prorocentrum clipeus cells were nearly circular in shape,with a collar,ridge,and one protrusion.The periflagellar area was wide U-shaped,with two curved projections on platelet 1a.Nine periflagellar platelets of different sizes were observed.The morphology closely fits that of the species isolated from other locations.Phylogenetic analysis based on the molecular sequences of the small subunit(SSU)rDNA,internal transcribed spacer(ITS),and large subunit(LSU)rDNA was performed.A comprehensive metabolomic analysis incorporating target,suspect and non-target screenings was first applied to investigate the intracellular and extracellular metabolite profiles of the current isolate of P.clipeus.According to the results of the target and suspect screenings,179 metabolites or toxins produced by DSP-related algal species,including OA,dinophysistoxin-1(DTX1),dinophysistoxin-2(DTX2)and pectenotoxin-2(PTX2),were not detected.Non-target screening involving feature-based molecular networking(FBMN)provided a global view of major metabolites produced by the P.clipeus DF128 strain and revealed 23 clusters belonging to at least 13 compound classes,with organometallic compounds,lipids and lipid-like molecules,phenylpropanoids and polyketides,and benzenoids as major types.To date,this is the first record of the characterization of P.clipeus in samples from Chinese waters.Our results support the wide distribution of epibenthic Prorocentrum species.

Prorocentrumtaxonomyphylogenymetabolite profilingYellow Sea

Ruifang Wang、Mengmeng Tong、Shiwen Zhou、Junjie Zheng、Wenguang Zhang、Xinfeng Dai、Douding Lu、Jiarong Hu、Tianze Leng、Qinglin Mu、Zhongyong Yan、Jiangning Zeng、Pengbin Wang

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Ocean College,Zhejiang University,Zhoushan 316021,China

Key Laboratory of Marine Ecosystem Dynamics,Second Institute of Oceanography,Ministry of Natural Resources,Hangzhou 310012,China

The State Key Laboratory of Marine Pollution,Department of Biomedical Sciences,City University of Hong Kong,Hong Kong SAR 999077,China

Zhejiang Marine Ecology and Environment Monitoring Center,Zhoushan 316000,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNatural science Foundation of Zhejiang ProvinceNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaProgram of Bureau of Science and Technology of Zhoushan GrantBasic Public Welfare Research Project of Zhejiang Province

4170619141961144013LY20D0600044167611141876139419061402019C81031LGC22B050032

2024

10.1007/s13131-024-2302-2

海洋学报(英文版)
中国海洋学会

海洋学报(英文版)

CSTPCD
影响因子：0.323
ISSN：0253-505X
年,卷(期)：2024.43(8)