Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments
The quantification and diversity of denitrifying bacteria in Liaohe Estuary sediments were studied. Denitrifying bacteria quantification was analysed by MPN-Griess in spring, summer and autumn, the quantification of denitrifying bacteria was the highest in spring and the lowest in summer, and gradually fall from the Liaohe Estury to coastal area. Most nitrite reductase genes(nirS) comparability was less than 95％ with Genbank sequence in sediments of the Liaohe Estury by PCR-RFLP, a majority of denitrifying bacteria belong to Proteobacteria, Firmicutes and Actinobacteria, thereinto, Proteobacteria was dominant.Using nitrite reductase genes(nitS) of international nine estuary and Liaohe Estuary sediments, phylogenetic tree was constructed, the species of denitrifying bacteria was evidently different by the clustering analysis. The study of denitrifying bacteria diversity will provide an important theory foundation for microorganism participation in denitrification and the nitric biological geochemistry circulation.
NETL
NSTL
万方数据
维普
