CO2驱动的海水酸化对菲律宾蛤仔组织、免疫和抗氧化酶活及转录水平的影响
Effects of CO2-driven seawater acidification on tissue,immune and antioxidant enzyme activity and transcription levels of Ruditapes philippinarum
林毅 1陈强 1周思顺 1孔鲁闽 1黄张帆1
作者信息
- 1. 集美大学 水产学院,福建 厦门 361021;福建省海洋渔业资源与生态环境重点实验室,福建 厦门 361021
- 折叠
摘要
随着CO2 的大量排放,海洋酸化效应不断加重,为探究未来海水酸化情况对菲律宾蛤仔产生的影响,设置对照组(pH为 8.1)和酸化组(pH为 7.7、7.1和 6.4),研究周期为 42 d,测定菲律宾蛤仔在酸化条件下组织结构、免疫和抗氧化酶活性的变化情况,以及在分子水平上产生的影响.结果表明:菲律宾蛤仔置于酸化海水环境中,鳃丝间距随pH的降低而扩大,鳃丝纤毛黏合,水管和外套膜外表皮褶皱逐渐加深;鳃组织中酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)活性变化情况为先降后升,碱性磷酸酶(AKP)活性各组变化趋势不同,总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和溶菌酶(LZM)活性趋势为先升后降;鳃和内脏团谷胱甘肽过氧化物酶(GSH-Px)活性变化规律皆为持续上升;内脏团组织中LZM活性变化趋势各不相同,ACP活性变动趋势为先降后升,AKP、SOD和CAT活性变化规律为先升后降,T-AOC趋势为持续下降;通过转录组的分析得到,鳃组织GO功能主要富集在DNA整合、膜的组成部分和RNA定向DNA聚合酶活性等条目中,KEGG通路主要富集在吞噬体和与蛋白合成的相关通路中.海水酸化使菲律宾蛤仔组织呈现不同程度的损伤,破坏其内环境稳态,改变其代谢水平和与免疫相关基因表达,引发菲律宾蛤仔染病乃至死亡的风险上升.
Abstract
The ocean acidification effect is increasing with the large amount of CO2 emissions.To investigate the effects of future seawater acidification on Ruditapes philippinarum,a control group(pH = 8.1)and acidification group(pH = 7.7,7.1 and 6.4)were set up for 42 days.The changes in tissue structure,immune and antioxidant en-zyme activities of Ruditapes philippinarum under acidification conditions were measured,as well as the effects pro-duced at the molecular level.The results show that when Ruditapes philippinarum are placed in an acidified seawa-ter environment,gill filament spacing expands with decreasing pH,gill filament cilia adhere,and the pipes and out-er epidermal folds of the mantle gradually deepen.The activities of acid phosphatase(ACP)and superoxide dis-mutase(SOD)in gill tissues show a pattern of decreasing followed by increasing.Alkaline phosphatase(AKP)activities exhibit different trends in each group.Total antioxidant capacity(T-AOC),catalase(CAT),and lysozyme(LZM)activities show a pattern of increasing followed by decreasing.Glutathione peroxidase(GSH-Px)activities in gill and visceral masses show a continuous increase.LZM activity in the viscera group displays varying trends,while ACP activity shows a decreasing and then increasing pattern.AKP,SOD,and CAT activities exhibited an in-creasing and then decreasing pattern,while T-AOC activity shows a continuous decrease.Analysis of the transcrip-tome reveals that the GO functions in gill tissue are mainly enriched in DNA integration,integral components of the membrane,and RNA-directed DNA polymerase activity,among others.The KEGG pathway analysis shows enrich-ment in the phagosome pathway and pathways related to protein processing in the endoplasmic reticulum.The acidi-fication of seawater caused varying degrees of damage to the tissues of Ruditapes philippinarum,disrupting its in-ternal environmental homeostasis and altering metabolic levels and immune-related gene expression,and led to an increased risk of disease and even death in Ruditapes philippinarum.
关键词
二氧化碳/菲律宾蛤仔/免疫/抗氧化/转录水平Key words
carbon dioxide/Ruditapes philippinarum/immunity/antioxidant/transcript levels引用本文复制引用
基金项目
福建省自然科学基金(2020J01668)
出版年
2024
NETL
NSTL
万方数据