Evaluation of benthic ecological quality status in the Yalu River Estuary based on environmental DNA
Investigating the potential for application of environmental DNA to identify benthic organisms for evalu-ating ecological quality status collection of 17 benthic samples from the Yalu River Estuary,environmental DNA and morphological identification and comparative analyses of the resulting ecological quality assessment indices(AMBI,BENTIX,Shannon-Wiener H',M-AMBI)were used,respectively.The results show:environmental DNA identification of organisms belonging to 10 class,16 orders,19 families,20 genera and 22 species,morphological identification of organisms belonging to 9 class,27 orders,43 families,55 genera and 57 species,with a total of 10 organisms;the two methods of identification yielded AMBI inter-index(R=0.428,p=0.043,y=0.32x+1.08),BENTIX inter-exponential(R=0.430,p=0.043,y=0.28x+3.59)significant consistency exists,while there was significant difference between the Shannon-Wiener H'indices;the two methods of identification yielded AMBI in-tergrade,M-AMBI intergrade higher similarity,51.02%,44.90%,respectively;the AMBI and M-AMBI grades from the two identification methods were more in line with the actual situation and evaluated the overall ecological quality status of the Yalu River Estuary as good.This study demonstrates that the identification of benthic organ-isms based on environmental DNA to evaluate the ecological quality status has a high potential for application in marine environmental monitoring surveys.