首页|基于环境DNA评价鸭绿江口底栖生态质量状况

基于环境DNA评价鸭绿江口底栖生态质量状况

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为研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,本研究采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学进行鉴定,并对所得生态质量评价指数(AMBI、BENTIX、香浓-维纳H'、M-AMBI)进行比较分析.结果显示:环境DNA鉴定生物隶属于10纲16目19科20属22种,形态学鉴定生物隶属于9纲27目43科55属57种,共有生物10种;两种鉴定方法得出的AMBI指数间(R=0.428,p=0.043,y=0.32x+1.08)、BENTIX 指数间(R=0.430,p=0.043,y=0.28x+3.59)存在显著一致性,而香浓-维纳H'指数间存在显著差异性;两种鉴定方法得出的AMBI等级间、M-AMBI等级间相似性较高,分别为51.02%、44.90%;两种鉴定方法得出的AMBI与M-AMBI等级更符合实际情况,且评价鸭绿江口整体生态质量状况为良.本研究表明,基于环境DNA鉴定底栖生物评价生态质量状况,在海洋环境监测调查中具有较高的应用潜力.
Evaluation of benthic ecological quality status in the Yalu River Estuary based on environmental DNA
Investigating the potential for application of environmental DNA to identify benthic organisms for evalu-ating ecological quality status collection of 17 benthic samples from the Yalu River Estuary,environmental DNA and morphological identification and comparative analyses of the resulting ecological quality assessment indices(AMBI,BENTIX,Shannon-Wiener H',M-AMBI)were used,respectively.The results show:environmental DNA identification of organisms belonging to 10 class,16 orders,19 families,20 genera and 22 species,morphological identification of organisms belonging to 9 class,27 orders,43 families,55 genera and 57 species,with a total of 10 organisms;the two methods of identification yielded AMBI inter-index(R=0.428,p=0.043,y=0.32x+1.08),BENTIX inter-exponential(R=0.430,p=0.043,y=0.28x+3.59)significant consistency exists,while there was significant difference between the Shannon-Wiener H'indices;the two methods of identification yielded AMBI in-tergrade,M-AMBI intergrade higher similarity,51.02%,44.90%,respectively;the AMBI and M-AMBI grades from the two identification methods were more in line with the actual situation and evaluated the overall ecological quality status of the Yalu River Estuary as good.This study demonstrates that the identification of benthic organ-isms based on environmental DNA to evaluate the ecological quality status has a high potential for application in marine environmental monitoring surveys.

environmental DNAbenthic organismecological quality

王桂营、李宏俊、邵魁双、刘滨玮、楚奇、叶金清

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生态环境部国家海洋环境监测中心,辽宁大连 116023

环境DNA 底栖生物 生态质量

业务化监测项目

2024

海洋学报(中文版)
中国海洋学会

海洋学报(中文版)

CSTPCD北大核心
影响因子:1.044
ISSN:0253-4193
年,卷(期):2024.46(3)
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