Microbial diversity of alkane-and plastic-degrading microbiome in offshore sediments of Ross Sea,Southern Ocean
Oil and plastic pollutants are a serious threat to marine ecosystems and have even been found in the Ross Sea of the Southern Ocean.In order to obtain low-temperature alkane degrading bacteria and plastic-degrading bac-teria in the region,a total of twelve sediment samples were collected in the Ross Sea area for enrichment and isola-tion of alkane-degrading bacteria at low-temperature,and the diversity analyses of the tetradecane-enriched com-munities showed that the most dominant genera were Pseudomonas,Alcanivorax,Marinomonas,Pseudoalteromo-nas.The polyethylene terephthalate(PET)and polyethylene(PE)was further validated using the dominant alkane-de-grading bacteria.Scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(ATR-FTIR)demonstrated that the four pure cultures of Pseudomonas pelagia R1-05-CR3,Pseudomonas taeanensis A11-04-CA4,Halomonas titanicae A11-02-7C2 and Rhodococcus cerastii R1-05-7C3 could degrade PE effectively.The results of UPLC-MS and SEM confirmed the PET degradation by isolates of R.cerastii R1-05-7C3,Microbacteri-um maritypicum RA1-00-CA1,and H.titanicae A11-02-7C2.In conclusion,this study reports the diversity of the tetradecane-enriched consortia at low-temperature and plastic degrading bacteria in the offshore sediments of the Ross Sea in the Southern Ocean,which play a selfpurifying role in in-situ environmental contamination,and also provided strain resources for the biodegradation of hydrocarbon and plastic contaminants at low temperature.
Ross Seaalkanespolyethylenepolyethylene terephthalatebiodegradationcold-adapted microorganisms
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